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Recent Activity
JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
RyanWheeler posted in Plaques appear on spot test, don't appear in titer
All posts created by debbie
| Link to this post | posted 15 Jun, 2022 20:08 | |
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Kristen, I do know it is recurring. But every time I have encountered it, folks re-enter and it then works. Did you exist DNA Master after you re-entered the info and before you reprocessed? Try that and see if it works. I am guessing at this point. debbie |
| Link to this post | posted 01 Jun, 2022 18:30 | |
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PineapplePizza is the first Microbacterium phage that is like phi29 (a Bacillus phage) and P68 (a Staphylococcus phage). I have used a new set of structural gene names for its various parts that are unique to phages like this. See the attached map for specifics. Cool little phage! debbie |
60Kb| Link to this post | posted 01 Jun, 2022 15:40 | |
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Annotate it as the data shows. New data is available all of the time, and we will try to keep up with it. This post is most helpful. I would recommend that you draw attention to this in your cover sheet, so that the reviewer doesn't take it out. Help them to benefit from what you investigated. Thanks! debbie |
Posted in: Cluster EF Annotation Tips → Small terminase
| Link to this post | posted 24 May, 2022 15:28 | |
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Hi all. Seems that the TAC ribosomal slippage is difficult to locate. Do not call the slippage at CCCGGAA. It is not a canonical choice. However, recognize that there are some members in Subcluster DN3 and DN4 (Apricot, for example) that do have a canonical slippage, namely G_GG.G_GA.A (where _ separates codons in the original frame, and . separates them in the new frame). |
Posted in: Cluster DN Annotation Tips → tail assembly chaperones
| Link to this post | posted 22 May, 2022 22:03 | |
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Tammy, The corrected file has been uploaded to phagesDB and sent to GenBank. Thanks, debbie |
| Link to this post | posted 22 May, 2022 01:50 | |
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Nope. Thanks for checking. We can correct this. debbie |
| Link to this post | posted 20 May, 2022 19:48 | |
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Nice! |
Posted in: Functional Annotation → Refining the call for Cas4 family exonuclease vs. RecE-like exonuclease
| Link to this post | posted 20 May, 2022 14:05 | |
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Yep. Please call them. Thanks, debbie |
Posted in: Functional Annotation → Membrane protein
| Link to this post | posted 19 May, 2022 17:21 | |
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Awesome. Do you log in to do your work? Just fyi - I always copy over input under a new id to submit my next sequence (then I don't have to pick the databases. debbie |
Posted in: Functional Annotation → HHPRED
| Link to this post | posted 18 May, 2022 13:18 | |
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Hi Sean, It should be back up this morning sometime. Steve Cresawn is aware of the problem. Thanks for checking in. debbie |
Posted in: Phamerator → Phamerator down?