Link to this post | posted 17 Jun, 2022 13:31

debbie

Hi all, As of today, June 17, 2022, there are 88 Cluster EE genomes in our records. They are closely related genomes that contain only ~28 genes. In an effort to make the records congruent, my students and I have reviewed ~75 of them and revised 71 of the records. The template we used is attached here, along with a list of 71 records that we touched. We spent an abundant amount of time on the helix-turn-helix DNA binding proteins at the right end of the genome. We investigated 'types' of helix-turn-helix choices and decided the best path is to call them helix-turn-helix DNA binding proteins. August 23, 2022 - I just received word that the genomes that we sent to GenBank have been processed. This cluster just might have congruent calls! Edited 23 Aug, 2022 16:13 74Kb 12Kb

Link to this post | posted 16 Jun, 2022 18:05
debbie	Kathleen and Chris, After reading Chris' entry, he is right -the slippage called in Satis, though it looks very slippery, is not canonical, i.e. not found in the literature. To that end, if we indeed are not calling any of the slippages, I would be inclined to NOT assign the function of a tail assembly chaperone (because there is no HHPred evidence of a TAC) unless I overlooked something.

Link to this post | posted 16 Jun, 2022 17:52

debbie

I just looked at the tail assembly chaperones of Cluster BM. Here is my take: currently there are six members: Satis Kradla EhyElimyoE JustBecause Kela Frankenweenie To the best of my knowledge none of the protein sequences in question here show an HHPred hit to a tail assembly chaperone (TAC). In order to call them TACs, if they possessed a canonical slippage, they could be called TACs and the slippage would be annotated. The ribosomal slippage found in Satis looks great, AND is identical to what is in Kradal and EhyElimyoE (though they are not called….. yet). Neither JustBecause or Kela have that same slippage. And Frankenweenie doesn't look at bit like the others in the c-terminus. (do a blastp - sequence alignment with Satis_58 and you will see how the alignment falls apart. However, the sequence homology with Satis is compelling. i would be inclined to call both genes TACs, but do not slip them together. I think that is what I would do. Good luck! debbie

Link to this post | posted 16 Jun, 2022 16:37
debbie	Welcome!

Link to this post | posted 15 Jun, 2022 23:27
debbie	Hi Kathy, We have a bit of evidence in mass spec data that says that the -1 start is the only one that is present. So in general, we are selecting the -1 choice over the -4 when both are present. debbie

Link to this post | posted 15 Jun, 2022 20:08
debbie	Kristen, I do know it is recurring. But every time I have encountered it, folks re-enter and it then works. Did you exist DNA Master after you re-entered the info and before you reprocessed? Try that and see if it works. I am guessing at this point. debbie

Link to this post | posted 01 Jun, 2022 18:30
debbie	PineapplePizza is the first Microbacterium phage that is like phi29 (a Bacillus phage) and P68 (a Staphylococcus phage). I have used a new set of structural gene names for its various parts that are unique to phages like this. See the attached map for specifics. Cool little phage! debbie 60Kb

Link to this post | posted 01 Jun, 2022 15:40
debbie	Annotate it as the data shows. New data is available all of the time, and we will try to keep up with it. This post is most helpful. I would recommend that you draw attention to this in your cover sheet, so that the reviewer doesn't take it out. Help them to benefit from what you investigated. Thanks! debbie Edited 01 Jun, 2022 15:40

Link to this post | posted 24 May, 2022 15:28
debbie	Hi all. Seems that the TAC ribosomal slippage is difficult to locate. Do not call the slippage at CCCGGAA. It is not a canonical choice. However, recognize that there are some members in Subcluster DN3 and DN4 (Apricot, for example) that do have a canonical slippage, namely G_GG.G_GA.A (where _ separates codons in the original frame, and . separates them in the new frame).

Link to this post | posted 22 May, 2022 22:03
debbie	Tammy, The corrected file has been uploaded to phagesDB and sent to GenBank. Thanks, debbie