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Recent Activity
All posts created by debbie
| Link to this post | posted 20 Feb, 2021 00:04 | |
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You're welcome! |
Posted in: Functional Annotation → PLA2-like domain in Cluster B1s
| Link to this post | posted 19 Feb, 2021 23:49 | |
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I do not think that this protein (gene35 of Sarma624) has a reportable function. HHPred has no hits to an applicable function. |
Posted in: Functional Annotation → PLA2-like domain in Cluster B1s
| Link to this post | posted 19 Feb, 2021 01:09 | |
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Yep. Looks like an error to me. We will get this corrected as we continue with genome curations. Thanks! debbie |
| Link to this post | posted 15 Feb, 2021 17:07 | |
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Joe, It doesn't matter, just state (maybe in your cover letter) what you used. debbie |
Posted in: Annotation → % alignment
| Link to this post | posted 09 Feb, 2021 23:26 | |
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Hi Greg, The jury is really out on all of this. We are in need of wet bench data, which is coming, but not here yet. There is just no answer at this time. I ran a comparison of the different settings and the new setting will work for now. An alternative when you run with the old settings is to parse the sequence into parts. I ran a 20kb length of a bigger genome and was able to run it at the previous setting easily. All i have for right now. Will update with additional data. a;ways check the Bioinformatics Guide for latest settings because I will keep it updated. Thanks, debbie |
Posted in: tRNAs → tRNAScan-SE Down-ish?
| Link to this post | posted 06 Feb, 2021 19:05 | |
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Hi all, The site is not broken. It just won't run in any timley fashion with the settings we have been using. We did some fiddling with our settings and have modified how to run tRNA Scan SE. Go to the Bioinformatics Guide to get them! |
Posted in: tRNAs → tRNAScan-SE Down-ish?
| Link to this post | posted 06 Feb, 2021 18:54 | |
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and I just revised the protocol again. |
Posted in: tRNAs → tRNAscan-SE site has changed?
| Link to this post | posted 27 Jan, 2021 19:20 | |
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Chris, Blast x takes a nucleotide sequence, translates it into all 6 reading frames and blasts. That is not needed if you want to know if the thing that was predicted by Glimmer and GeneMark has any homologues. tBlastx takes too long and would just be confusing, coding potential provides you the ORF you want to zero in on. If at any time, you think you need to look elsewhere - in another reading frame- go for it. Using nucleotide sequences to look for function homologues is not nearly as helpful as using the protein sequence. The ORFs identified by Glimmer and GeneMark (and in your auto-annoted DNA Master file) have pushed past looking for protein homologues across all 6 reading frames and showing the most likely ORF with coding potential (those are powerful and proven algorithms. Blastx of any kind is not routinely done. It can be used when you are just not convinced of what you are finding. Hope that helps, debbie |
Posted in: DNA Master → DNA Master Blast type
| Link to this post | posted 26 Jan, 2021 00:57 | |
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Hi. I typically use the phage trained GeneMark in PECAAN, so no harm. Be sure to label the file so you know which one it is. BUT I use the host(s) trained outside of PECAAN because all can be informative, especially in the difficult to call areas. debbie |
Posted in: PECAAN → GeneMarkS ?
| Link to this post | posted 26 Jan, 2021 00:38 | |
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Hi Greg, I am not having trouble blasting. (I only blasted 1 gene but it completed in less than 3 minutes. I would guess that this is a server/firewall sort of problem. I would recommend restarting your computer then calling your IT folks. debbie |