Link to this post | posted 18 Nov, 2022 02:40
debbie	Cluster EC genomes may have 2 capsid proteins. See the note at Cluster DC. https://seaphages.org/forums/topic/5430/?page=1#post-9831 Edited 18 Nov, 2022 02:42

Link to this post | posted 18 Nov, 2022 02:35

debbie

Simon White's CroEM work has discovered that the major capsid in some of our genomes is made with 2 genes - a major capsid pentamer protein and a major capsid hexamer protein in Rosebush (Cluster B2). Cluster DC genome have the same hexamer protein and a homolog of the pentamer protein. Examples are: Wizard gp 19 - major capsid hexamer protein Wizard gp 20 - major capsid pentamer protein Edited 18 Nov, 2022 02:36 2.94Mb

Link to this post | posted 14 Nov, 2022 16:55
debbie	I think that this is a thymidylate kinase.

Link to this post | posted 11 Nov, 2022 03:00
debbie	Louise, Here is what has worked in the past. See if it still works. debbie https://seaphages.org/forums/topic/4795/

Link to this post | posted 04 Oct, 2022 16:37
debbie	If further info is needed, pictures would be helpful. Good luck!

Link to this post | posted 04 Oct, 2022 15:45

debbie

Hi Stephanie, If you are using 7H10 to make your agar, it contains malachite green and the agar should really be green. Once overcooked and autoclaved, it tends to be more tan than pale green. If this is the cause of the green, there is nothing wrong. If the agar is undercooked or autoclaved, it can appear greener, BUT you will also have no growth and/or contamination issues, which are a bigger problem. Does that fit what you are seeing?

Link to this post | posted 13 Sep, 2022 13:22

debbie

Tammy, We have used Corynebacterium vitaeruminis a bit. I believe we used PYCa system for plating. My recollection is that the original strain has a prophage in it, the Corynebacterium vitaeruminis CAG1 strain is cured of its prophage. Denise Monti has the most experience, so I would ask her. (montidenise2@gmail.com) We have not obtained any NRRL Corynebacterium hosts, but they look like they have a few. Good luck! Keep us posted on how you proceed! best, debbie

Link to this post | posted 12 Sep, 2022 17:25

debbie

Hi Alison, Could it be that you have a mutant of "WT" Arthrobacter sp.? So you still have your original stock in the freezer? You will want to use that until you confirm what the orange culture is. (Maybe a 16S to confirm). I believe G. terrae CAG3 is a mutant of G. terrae 3612, and is also a mutant in the gene that produces the color. In order to use it for phagehunting, you will want to confirm what you have. With propagation, does it stay orange? or does it go back to yellow. (Is this a mutable change or an environmental change?) Did you make any changes in the prep materials (a different brand of agar)? You will want to test your previous phages on the the new host to see if this change is results in a different infection pattern. If it is now a different bacteria than you started with, meaning it is an inherited trait, it is a new strain, subspecies and should be labeled as a new thing. Keep us at Pitt in the loop so that you enter your phage information correctly. We can add a new subspecies to the list if this proves to be true. Show us a picture of the pretty orange! debbie

Link to this post | posted 19 Aug, 2022 23:36
debbie	To the best of knowledge, DNA mater will not work on an M1 Mac. debbie

Link to this post | posted 12 Aug, 2022 15:33
debbie	Hi Amanda, I see no clear evidence for calling this one anything but a Hypothetical Protein. (My best explanation for why some members of the pham are called terminase, small subunit is there was a moment in time when we thought we could call by synteny. But I would not recommend that today.) Best, debbie