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Recent Activity
All posts created by debbie
Link to this post | posted 16 Jun, 2022 16:37 | |
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Welcome! |
Link to this post | posted 15 Jun, 2022 23:27 | |
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Hi Kathy, We have a bit of evidence in mass spec data that says that the -1 start is the only one that is present. So in general, we are selecting the -1 choice over the -4 when both are present. debbie |
Link to this post | posted 15 Jun, 2022 20:08 | |
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Kristen, I do know it is recurring. But every time I have encountered it, folks re-enter and it then works. Did you exist DNA Master after you re-entered the info and before you reprocessed? Try that and see if it works. I am guessing at this point. debbie |
Link to this post | posted 01 Jun, 2022 18:30 | |
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PineapplePizza is the first Microbacterium phage that is like phi29 (a Bacillus phage) and P68 (a Staphylococcus phage). I have used a new set of structural gene names for its various parts that are unique to phages like this. See the attached map for specifics. Cool little phage! debbie |
Link to this post | posted 01 Jun, 2022 15:40 | |
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Annotate it as the data shows. New data is available all of the time, and we will try to keep up with it. This post is most helpful. I would recommend that you draw attention to this in your cover sheet, so that the reviewer doesn't take it out. Help them to benefit from what you investigated. Thanks! debbie |
Posted in: Cluster EF Annotation Tips → Small terminase
Link to this post | posted 24 May, 2022 15:28 | |
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Hi all. Seems that the TAC ribosomal slippage is difficult to locate. Do not call the slippage at CCCGGAA. It is not a canonical choice. However, recognize that there are some members in Subcluster DN3 and DN4 (Apricot, for example) that do have a canonical slippage, namely G_GG.G_GA.A (where _ separates codons in the original frame, and . separates them in the new frame). |
Posted in: Cluster DN Annotation Tips → tail assembly chaperones
Link to this post | posted 22 May, 2022 22:03 | |
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Tammy, The corrected file has been uploaded to phagesDB and sent to GenBank. Thanks, debbie |
Link to this post | posted 22 May, 2022 01:50 | |
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Nope. Thanks for checking. We can correct this. debbie |
Link to this post | posted 20 May, 2022 19:48 | |
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Nice! |
Posted in: Functional Annotation → Refining the call for Cas4 family exonuclease vs. RecE-like exonuclease
Link to this post | posted 20 May, 2022 14:05 | |
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Yep. Please call them. Thanks, debbie |
Posted in: Functional Annotation → Membrane protein