Link to this post | posted 02 Oct, 2025 01:05
debbie	HI Brian, When I look at the Starterator report, I see one common start for every phage listed - it is technically threaded through the starts listed as 9, 10 and 11. Which is the start that corresponds to bp 110. Remember that starterator is a clustal alignment and as the sequence diverges, small discrepancies at the nucleotide level can be present. I would call 110 as the start. debbie

Link to this post | posted 24 Sep, 2025 18:59
debbie	Hi Allie, Yes, i think that Feature 89 (50477-50701bp) and Feature 91 (50689-50862bp) have more evidence to support keeping them than Feature 90R (50695-50546bp). I would delete Feature 90R (50695-50546bp). Best, debbie

Link to this post | posted 22 Sep, 2025 17:13

debbie

Hi Allie, I would keep it. I particularly like the coding potential (those slight) when using M. tb as the model. I am also not as concerned about the overlap, because it is the c-terminus of both genes that overlap, meaning that as they get transcribed/translated, the process started upstream and went through to completion. there is no upstream sequence needed to get their translation/transcription started. Note too, that the sequence is well-conserved across other clusters, even though these are tiny little genes. What do you think? debbie

Link to this post | posted 18 Sep, 2025 15:29

debbie

Hi Beth, That is a good question. I recommend that you only post single purified phages on phagesDB and archive those with us. What do do with 'other' samples that are not purified is really up to you. To save stuff for a future date, it then becomes a storage and record keeping issue. If you are asking how to store them, that depends on volume. Storing phages in high enough volumes in the frig will work for a while (likely years), and very host dependent. You can add 7% DMSO to the samples and store at 4C. Hope that helps! debbie

Link to this post | posted 03 Sep, 2025 23:36
debbie	fixed!

Link to this post | posted 02 Sep, 2025 13:23

debbie

Kissaou, The power of comparative genomics will aid in this quest to see if it is plausible that a gene is present in this area. If there an open reading frame that looks plausible. you can blast n and blast p the area. if this area is highly conserved, what are the possible explanations? If it is not conserved, what does that tell you. Check out the dates of the genomes that have this sequence. What have they called. What is the evidence on which they made their calls. That data is all present in the tools that you have access to. Lots of information, tricky decision. let us know what you find out. debbie

Link to this post | posted 02 Sep, 2025 13:18

debbie

Kissaou from Austin Community college wrote: I am kissaou , and I am making this post regarind Mycobacterium phage "Teodoridan" Cluster A1 we completed the annotation, but I am a little it not happy about the 311 bp between gene 2 and gene 3. Any idea? Looking at DNA master frames, it is possible to inser a gene between Start 1474bp - stop 1665bp. However, looking at glimmer and genemarks, there is not a reall coding potential. What everybody else think? Thanks Kissaou

Link to this post | posted 28 Aug, 2025 14:24
debbie	Ombeline, Hi. I don't have time to troubleshoot, but I will make your profiles for you. Would you send me well labeled files and I will get them back to you? Best, debbie

Link to this post | posted 14 Aug, 2025 17:29
debbie	Rick, Very nice. Also a very fun area to think about. Cluster FK are not the only ones to have these. Cluster Bs, and Os also. Any categorization of these would be helpful! debbie

Link to this post | posted 11 Aug, 2025 15:16

debbie

Hi Randy, This is going to take a bit of time to check. And i don't have that time, so maybe I can provide enough info' to get you started. Historically, it was thought that all sipho have TACS, canonically directly upstream of the tape measure. There is now reports that the genes are present, and do not have to be beside each other. And that not all sipho have TACs. There is also evidence that the 4 nucleotide sequence of XXXY can constitute a slippage (if they are in the 'right spacing' of the reading frames). That is part of the evaluation that can occur. Having the numerous cluster members to compare will also be informative. I don't know what you meant about a second slippage site. You are correct to look more closely. debbie