Link to this post | posted 04 Jan, 2017 18:06
debbie	Here is the list of phages for the host range project and their titers. Note: Wintermute is a phage found by Bryan Pasqualucci at Southern Connecticut State University (incorrectly labeled in the sheet that was sent with the lysates). Sorry Bryan! 20Kb

Link to this post | posted 03 Jan, 2017 16:33
debbie	Roy, Happy New Year! I sent an email to Dr. Lawrence and he rebooted his website this AM. I found it to be working now. Thanks for the heads up, debbie

Link to this post | posted 16 Dec, 2016 20:33

debbie

PECAAN (Phage Evidence Collection and Annotation Network) Claire Rinehart, from Western Kentucky University, introduced us to PECAAN at last summer’s Retreat. PECAAN is a companion program to DNA Master (not a replacement), streamlining the complicated decision-making process of Gene Prediction by displaying relevant evidence for each gene on its own web page. It also provides a record-keeping system that can be used as input to create your final DNA Master files. Using PECAAN is optional. You may want to add PECAAN into your students’ workflow once they get the hang of using DNA Master and evaluating the data from the various data sources. Or you may want to use it as a tool for the final review of your genomes because it stores BLAST (PhagesDB and NCBI), HHPRED, and Starterator data for the entire genome and can output this data into DNA Master as a functional file or as a fully annotated file. To gain access to PECAAN, instructors should email claire.rinehart@wku.edu a request along with:     Firstname, MI, Lastname     Email     Full institution name     Approximate number of students in their group. The PECAAN user manual is available on seaphages.org on the Faculty Information page. The user manual contains instructions on how to load phages into PECAAN and how to add your students as users of the site. Edited 16 Dec, 2016 20:34

Link to this post | posted 16 Dec, 2016 17:12
debbie	Katie, Hi. I just posted a new topic on the Xeno project so you can record results there. Thanks!

Link to this post | posted 16 Dec, 2016 17:04
debbie	Hi all. After you have completed the spreadsheet for your results, you can post it here and complete the summary data on the Google spreadsheet. I just changed access to this spreadsheet so you can now edit it.

Link to this post | posted 16 Dec, 2016 16:17
debbie	As you record your host range data, please upload your Excel sheets to this post! I also just changed setting on the summary Google spreadsheet so you can add your summary data there! Edited 16 Dec, 2016 17:08

Link to this post | posted 25 Oct, 2016 20:07
debbie	Jeremy, If you join the program (and go through our training) we set up an MTA between U of Pittsburgh and your school to be able to ship M. smegmatis mc2 155 to you. So you could use your bacteria to pilot and then you will have smeg by August of next year (assuming you are accepted into the program).

Link to this post | posted 22 Oct, 2016 19:35
debbie	The Hatfull Lab Undergraduate researchers prepared the lysates for the Host Range project. The leaders of the group are pictured here: (from left to right) Audrey, Patrick, Gabby, and Johnathon. Edited 01 Nov, 2016 15:56 184Kb

Link to this post | posted 18 Oct, 2016 01:14
debbie	Follow the Sensitivity Testing Protocols as described in the Phage Discovery Guide (Protocol 11.4) and outlined in the attachment in the Basic Information post. Be sure to read the Cluster N paper! Coming Soon! If you have questions about the protocols, use this topic. Thanks, debbie

Link to this post | posted 18 Oct, 2016 01:07

debbie

The results of the Xeno Project will be documented on this Forum and will consist of two parts: 1. Summary Report Use this spreadsheet to record a summary of the results of your testing. I have included testing done in the Hatfull lab as an example. You can add additional phages and additional schools to the list as needed. 2. Data Sheets The attached sheet - Xeno Template - will provide you with a template to include all the necessary data to make sound interpretations of that data. Instructions are on the sheet. Review this data sheet as you perform these experiments. In addition to the template, an example,Hatfull lab data, is also attached. Edited 18 Oct, 2016 01:13 46Kb 1.89Mb