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JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
RyanWheeler posted in Plaques appear on spot test, don't appear in titer
All posts created by debbie
Link to this post | posted 04 Mar, 2020 19:42 | |
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Success! always a good day! debbie |
Link to this post | posted 04 Mar, 2020 18:16 | |
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Bob, The simple answer is that yes, the tape measure in Magel is in two pieces. However, that does not imply a programmed frameshift as we see in the tail assembly chaperone genes. Much bench work would be needed to confirm that there is a ribosomal slippage. It would be great to say that the upstream gene is a particular domain of the tape measure gene, but I don't think that is obvious. However more investigation could help to elucidate if the upstream gene has a particular function that we could identify. debbie |
Link to this post | posted 04 Mar, 2020 17:31 | |
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Maria, I just took your file and generated a GenBank file with no errors. Did you choose the "Bacteria and Plant Plastid Code" on the Description pane of Submit to GenBank? all of the genes you have errors for have GTG starts (I think). debbie |
Link to this post | posted 03 Mar, 2020 17:50 | |
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Hi all, I am going to chime in here, but my understanding today is that every phage may not have the G/T programmed frameshift. In lots of cases we can only find homologues to G and nothing to T. If the downstream gene of the frameshift does not have homologues and you cannot find a slippery sequence as denoted in the review paper cited in the guide https://seaphagesbioinformatics.helpdocsonline.com/article-54 , do not 'force' a frameshift. I would not call 4Gs, not followed by 3 of something else as a slippery sequence (though we have done it in the past). Is that the case here? debbie |
Link to this post | posted 01 Mar, 2020 13:01 | |
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Hi Greg and all, Delete the 2 N's in the sequence pane of the file. Then click the "Raw" button (top right corner). I could try to tell you what "Raw" means, but I do not know the origin. What this button does is some form of reformatting to the sequence (to endure there is no 'hidden' characters) that allows the change to be posted. (Also make sure that the little 'lock' icon in the bottom right corner is 'open (unlocked). debbie |
Link to this post | posted 28 Feb, 2020 02:28 | |
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Greg, Here are my best answers: Is there a way to align the two DNA sequence files to determine where the nucleotides have been added or deleted? Yes! Use the align 2 sequences at ncbi's blastn. But my best guess is that there are 2 Ns at the end of the longer sequence. i would check there first. |
Link to this post | posted 19 Feb, 2020 18:05 | |
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Just looked at 2 cluster AO2 phages, JKerns and StevieBAY. StevieBAY's gene 28 is an endolysin. It possesses all of domains we expect to see in an endolysin. Gene 64 codes for the CHAP endopeptidase domain of LysK (a Staphylococcus phage lysin gene). It is reported out as an endolysin also. We won't call either of them lysin A (Reserved only for mycobacteriophages for now, unless the phage genomes contains a lysin A and a lysin B. |
Link to this post | posted 12 Feb, 2020 18:45 | |
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Deb, The file (not the program) is likely corrupted. Follow these directions in the Bioinformatics Guide. https://seaphagesbioinformatics.helpdocsonline.com/article-104 It is tedious, so be careful as you do this. If it doesn't work, send me the file. Good luck! debbie |
Link to this post | posted 05 Feb, 2020 21:09 | |
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Excellent idea! Thanks Chris! |
Link to this post | posted 02 Feb, 2020 20:00 | |
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Amy, If send me the file, I will try to fix it. debbie |