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All posts created by debbie

| posted 25 Jan, 2021 13:23
Hi Steve,
https://seaphages.org/forums/topic/224/
This link will take you to PECAAN‘s user guide. It provides directions for you to add your phage to PECAAN.
Thanks,
debbie
Posted in: Bioinformatic Tools and AnalysesPECAAN
| posted 24 Jan, 2021 12:08
Maria,
without evidence, Hypothetical Protein is the best choice.
thanks,
debbie
Posted in: Functional AnnotationSingleton FuzzBuster Major Capsid
| posted 12 Jan, 2021 20:34
Andrea,
That is just incorrect. Gene 21 in ActinUp is the tape measure.
debbie
Posted in: AnnotationTape measure in K1 phage
| posted 08 Jan, 2021 00:33
Hi all,
And remember that Holliday is named after a person, so it is one of the few words that we capitalize. "In 1964, Robin Holliday proposed a model to explain three important events that occur during meiosis in fungi: crossing-over, gene conversion, and postmeiotic segregation." (Holliday Junction Resolvases,Cold Spring Harb Perspect Biol. 2014 Sep; 6(9): a023192.
doi: 10.1101/cshperspect.a023192)
Posted in: Functional AnnotationRuvC resolvase vs Holliday Junction Resolvase
| posted 05 Jan, 2021 14:10
Fernando,
There are a lot of factors that impact how poorly Blast completes. It sounds like it just needs to have more time. I have waited longer than overnight to complete a blast. You can try blasting in sections. See this section of the guide.
https://seaphagesbioinformatics.helpdocsonline.com/article-70

good luck!
debbie
Posted in: DNA MasterDNAMaster BLAST failure
| posted 30 Dec, 2020 23:44
Matt,
Ok. It is never simple, is it?
Good luck.
debbie
Posted in: DNA MasterDNA Master Application Corrupted
| posted 30 Dec, 2020 20:04
Hi Matt,
This sounds like 2 different problems, both alluding to incomplete download/installation problems. I would first recommend uninstall and reinstall.
Also, does the installer have admin priviledges? You are missing some files/drivers. Do you still have DNA master on your old computer? We may have to do some copy/pasting.
debbie
Posted in: DNA MasterDNA Master Application Corrupted
| posted 17 Dec, 2020 02:48
Dr. Catalase,
Yes. Please use the validation function in DNA Master to do this when you renumber the genes. It is always best to let DNA Master auto-fill any field that you can
debbie
Posted in: Notes and Final FilesFilling in the Product field
| posted 15 Dec, 2020 21:24
Chris,
You are the best!
thanks,
debbie
Posted in: StarteratorRead First: Common Starterator Troubleshooting
| posted 15 Dec, 2020 13:17
Juan,
GpXX is incorrect. You should use the buttons of DNA Master to overwrite gpXX with Hypothetical Protein. The function field is empty. The Bioinformatic guide should be followed.

CDS 379 - 1161
/gene="1"
/product="Hypothetical Protein"
/locus tag="SEA_MYPHAGE_1"

Good luck!

debbie
Posted in: Notes and Final FilesHypothetical Proteins in the Product Field