Link to this post | posted 03 Jun, 2021 21:35

debbie

Hi Kathleen, I am no expert, but the error is because DNA Master was written before the era of secure https. I believe that you are missing some SSL libraries. I had referred to these in the past, but I am not confident that they work. https://www.dropbox.com/sh/mtvey9ohuuykv7r/AADuZje-yHWf1SIRDE0eg9gVa?dl=0 His issue was then, where to put them. They are located in the main DNA Master folder on my computers (in Program files), so put them there. But, if that doesn't work or you like his advice better, here is another option. Christian Gauthier found the right stuff that worked for him here. For your future reference on this point, a lightweight program that folks can install to fix this issue is the Bitvise SSH Client (https://www.bitvise.com/ssh-client-download). Apparently it puts everything in the right place because DNA Master is now performing remote BLAST jobs without complaint! Fingers crossed! debbie Edited 03 Jun, 2021 21:36

Link to this post | posted 01 Jun, 2021 10:32
debbie	Hi Kari, Short and repetitive sequences are the culprits; some of the default settings for QBlast are probably different might exclude too much. That would lead to a very high EValue threshold, so no Results. Very little in QBLAST is specified by the user. In order words, it is an internal DNA Master setting. Just note that DNA Master wouldn't Blast it on your cover sheet. Thanks, debbie

Link to this post | posted 27 May, 2021 15:20
debbie	Terrific! Thank you!

Link to this post | posted 27 May, 2021 00:18
debbie	Hi Joe, Sorry, that is my fault. I didn't mean to make it confusing. I was thinking about the minimal file and you are talking about the complete notes file. The rationale for using is the function field is so that the product field can be deleted and then re-populated from the function field. I hope that makes sense. debbie

Link to this post | posted 27 May, 2021 00:16
debbie	Becky, I asked around because I don't use Wine. Tammy Adair (at Baylor) offered this: "As far as I can tell we cannot use wine on any systems past Catalina . Most of my Mac students used a virtual machine set up that our IT department provided. The IT guy that I have been working with for the past 10 years does not see a fix.".

Link to this post | posted 26 May, 2021 22:31
debbie	Hi Kathleen, Did you shutdown the computer and restart? debbie

Link to this post | posted 26 May, 2021 21:34
debbie	Joe, We do not use function field. We record function in the product field and you can auto-populate the fields of your choice by using the arrow that is in the upper right corner of each field. Make sense? debbie

Link to this post | posted 26 May, 2021 01:46
debbie	Hi Kathleen, Sounds like your preferences are not quite right. https://seaphagesbioinformatics.helpdocsonline.com/article-66 In step 2, make sure that you have the server set correctly. the unlock code is Hatfull and there is no password. debbie

Link to this post | posted 25 May, 2021 17:28
debbie	Hi Nikki, I am sure I called CrystalP, and now want to disagree with the intron part of that call. According to the literature, the introns have to be self-splicing in a prokaryote. So it has to be ~200-ish nucleotides. As for whether that second tRNA is worth calling, the argument has persisted for 17 years. I need to investigate further. Give me a bit of time for this one. thanks, debbie

Link to this post | posted 25 May, 2021 16:58
debbie	Hurray!