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All posts created by debbie

| posted 26 May, 2021 22:31
Hi Kathleen,
Did you shutdown the computer and restart?
debbie
Posted in: DNA MasterMac errors
| posted 26 May, 2021 21:34
Joe,
We do not use function field.
We record function in the product field and you can auto-populate the fields of your choice by using the arrow that is in the upper right corner of each field.
Make sense?
debbie
Posted in: DNA MasterFunction field
| posted 26 May, 2021 01:46
Hi Kathleen,
Sounds like your preferences are not quite right.
https://seaphagesbioinformatics.helpdocsonline.com/article-66

In step 2, make sure that you have the server set correctly. the unlock code is Hatfull and there is no password.

debbie
Posted in: DNA MasterImportant DNA Master Update
| posted 25 May, 2021 17:28
Hi Nikki,
I am sure I called CrystalP, and now want to disagree with the intron part of that call. According to the literature, the introns have to be self-splicing in a prokaryote. So it has to be ~200-ish nucleotides.
As for whether that second tRNA is worth calling, the argument has persisted for 17 years. I need to investigate further. Give me a bit of time for this one.
thanks,
debbie
Posted in: tRNAsshort introns in tRNAs in cluster E
| posted 25 May, 2021 16:58
Hurray!
Posted in: DNA MasterBLAST won't save
| posted 24 May, 2021 20:08
Kathleen,
I just tried. My mac asked me to choose an application to open the ftp link. I clicked the "always" box and it downloaded. (see message screen shot). I did not overtly select an application.
Posted in: DNA MasterDNA master server down?
| posted 24 May, 2021 14:13
Nikki,
I have not encountered that. Here are some thoughts about it.
what is the size of the blasted file. Is it the size of a blasted or unblasted file (meaning is it 50kb or 250 kb-ish?)
If it is bigger, then the blast date is there. I am guessing that if you unpack and repack the .dnam5 file, it will 'appear".
https://seaphagesbioinformatics.helpdocsonline.com/article-104
what you will have do differently is go into the blast folder to make sure you have all the files.
Not sure it is the problem, but worth looking in to.
debbie
Posted in: DNA MasterBLAST won't save
| posted 20 May, 2021 19:52
It does now.
Sorry about the smile snafu! i am getting notices every time you try to fix it.
Posted in: Request a new function on the SEA-PHAGES official listantitoxin in toxin/antitoxin system, BrnA-like
| posted 07 May, 2021 20:42
You too!
Terrific that you contacted Kirk!
Thanks,
debbie
Posted in: Frameshifts and Intronslysin A gene split with an intron?
| posted 07 May, 2021 20:03
Hi Andrea,
i do not know how to do this in PECAAN, but in following Kirk's explanation for Coco12, i would annotate it the same way he did.
debbie
Posted in: Frameshifts and Intronslysin A gene split with an intron?