Link to this post | posted 19 Jan, 2024 18:11
debbie	Hi Johann, The header of the fasta file for Franklin22 had a misspelling in it, so it read Frankin22. If you go to Franklin22's page, you can get the info that you need. The header has been corrected, but will not post until sometime later tonight or tomorrow. best, debbie

Link to this post | posted 03 Jan, 2024 12:23
debbie	The most current version of DNA Master is 5.23.6, build 2705, 24 October 2021. Make sure preferences are set correctly, check #6, to make sure that the sftp settings are correct). Then, in preferences, go to "Timed Events' and uncheck the first box. It does not need to check for updates again.

Link to this post | posted 18 Dec, 2023 02:22
debbie	Hi Fred, I would call 28 a holin and 29 a membrane protein. debbie

Link to this post | posted 10 Dec, 2023 01:58
debbie	The 2011 paper describes some repeat sequences in the right arm of The analyzed phages. In annotating phage Feyre, repeats were detected. See attached. 2Kb

Link to this post | posted 08 Dec, 2023 00:12
debbie	The transposase interrupts the N terminus of the immunity repressor (gp 43), and does not appear to be expressed (preliminary testing in the Hatfull lab). Therefore no immunity repressor gene has been called. Some details are in the attached document. 12Kb

Link to this post | posted 08 Dec, 2023 00:06
debbie	The immunity repressor in Feyre (gp 43) is interrupted by the insertion of the transposase. Preliminary testing shows that Feyre does not make a lysogen (and the immunity repressor is not working).

Link to this post | posted 01 Dec, 2023 04:59

debbie

More papers about cluster K phages: Mycobacteriophage ZoeJ: A broad host-range close relative of mycobacteriophage TM4 https://pubmed.ncbi.nlm.nih.gov/30948168/ A peptidoglycan hydrolase motif within the mycobacteriophage TM4 tape measure protein promotes efficient infection of stationary phase cells https://pubmed.ncbi.nlm.nih.gov/17083467/ Mycobacteriophage TM4: genome structure and gene expression https://pubmed.ncbi.nlm.nih.gov/10645443/ Genome Sequences of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, DarthP, Hammy, Krueger, LastHope, Peanam, PhelpsODU, Phrank, SirPhilip, Slimphazie, and Unicorn https://pubmed.ncbi.nlm.nih.gov/29217790/ Genome Sequences of Mycobacteriophages Findley, Hurricane, and TBond007. https://pubmed.ncbi.nlm.nih.gov/29122862/ Engineered bacteriophages for treatment of a patient with a disseminated drug-resistant Mycobacterium abscessus https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6557439/ Edited 01 Dec, 2023 05:05

Link to this post | posted 05 Oct, 2023 17:48
debbie	Hi all, I chatted with Graham about this and here's what i have gleaned from the conversation. Temperature and pH can affect how well the calcium goes into the solution. Maybe try a different source of peptone or yeast extract to see if it improves. best, debbie

Link to this post | posted 05 Oct, 2023 15:16
debbie	Eric, Yep. Calcium does precipitate out of solution at high temps. You can omit Ca in the autoclaved recipe and then add it back as you use the agar. You can also play with the concentrations. What you can't do is omit calcium. Many phages need calcium for adsorption, infection and stability. Make sense? debbie

Link to this post | posted 28 Sep, 2023 17:02

debbie

Hi Dane, In general, we keep one. But until the 'one' has been verified, meaning that the lysogen infection pattern 1) shows homoimmunity to the phage and 2)the phage is detected in the supernatant of the lysogen, more than 1 is advisable. The answer is also dependent on how the different streaks were obtained - from single colonies or out of a 'mesa', because having 4 different clones would be advisable depending on what you want to do. So what questions are you going to investigate with your newly made lysogens? Best, debbie