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Recent Activity
JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
RyanWheeler posted in Plaques appear on spot test, don't appear in titer
All posts created by debbie
| Link to this post | posted 13 Dec, 2022 02:04 | |
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2022 Genomics Workshop Participants, The genome we will annotate is Mycobacterium phage Ciao. Please add your annotation questions to this forum post. You will find Ciao in Phamerator and PECAAN. Be sure to identify the stop of the gene that you are interested in (it is the only unique identifier). Enjoy annotating! |
| Link to this post | posted 07 Dec, 2022 16:11 | |
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Working on Microbacterium phage Jefe at the 2022 Genomics Workshop! It is one of the members of this cluster where no slippery sequence can be identified. The upstream gene of the TAC pair has a Pfam hit to tail assembly chaperone, so call it. However, there is no data to support a tail assembly chaperone call for the second gene, NOR its there a slippery sequence present. that gene is a Hypothetical Protein. |
| Link to this post | posted 23 Nov, 2022 14:52 | |
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Hi Nikki, I think the Starterator picture shows that the 25523 is a best call. You can see it line up across different clusters/subclusters. I would call 25523. debbie |
| Link to this post | posted 18 Nov, 2022 02:45 | |
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Cluster GC genomes may have 2 capsid proteins. See the note at Cluster DC. https://seaphages.org/forums/topic/5430/?page=1#post-9831 |
Posted in: Cluster GC Annotation tips → 2 major capsid proteins
| Link to this post | posted 18 Nov, 2022 02:43 | |
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Cluster DE5 genomes may have 2 capsid proteins. See the note at Cluster DC. https://seaphages.org/forums/topic/5430/?page=1#post-9831 |
Posted in: Cluster DE Annotation Tips → 2 major capsid proteins
| Link to this post | posted 18 Nov, 2022 02:40 | |
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Cluster EC genomes may have 2 capsid proteins. See the note at Cluster DC. https://seaphages.org/forums/topic/5430/?page=1#post-9831 |
Posted in: Cluster EC Annotation Tips → 2 major capsid proteins
| Link to this post | posted 18 Nov, 2022 02:35 | |
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Simon White's CroEM work has discovered that the major capsid in some of our genomes is made with 2 genes - a major capsid pentamer protein and a major capsid hexamer protein in Rosebush (Cluster B2). Cluster DC genome have the same hexamer protein and a homolog of the pentamer protein. Examples are: Wizard gp 19 - major capsid hexamer protein Wizard gp 20 - major capsid pentamer protein |
2.94MbPosted in: Cluster DC Annotation Tips → 2 major capsid proteins
| Link to this post | posted 14 Nov, 2022 16:55 | |
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I think that this is a thymidylate kinase. |
| Link to this post | posted 11 Nov, 2022 03:00 | |
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Louise, Here is what has worked in the past. See if it still works. debbie https://seaphages.org/forums/topic/4795/ |
Posted in: DNA Master → DNA Master errors
| Link to this post | posted 04 Oct, 2022 16:37 | |
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If further info is needed, pictures would be helpful. Good luck! |
Posted in: Phage Biology → why is my agar green?