Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.
Recent Activity
Viknesh Sivanathan posted in 260/280 ratios too high from DNA extraction
lmeadows@rcbc.edu posted in 260/280 ratios too high from DNA extraction
nic.vega posted in 260/280 ratios too high from DNA extraction
Debbie Jacobs-Sera posted in Growing Liquid Cultures for lysogens
lmeadows@rcbc.edu posted in 260/280 ratios too high from DNA extraction
Integrases/Partitioning Systems
| Link to this post | posted 16 Jun, 2023 12:41 | |
|---|---|
|
|
Cluster A phages can have tyrosine, serine integrases, or partitioning genes. For the serine integrates, see the note about Bxb1 starts. Serine recombinases are called jumbo integrases because their size hovers around 500 amino acids (can go up over 700 amino acids). Look for serine residues in the N-terminus. Historically we didn't differentiate serine integrases from tyrosine integrases. Ask for help if you can't tell which one is in your genome. Some Cluster A phages, (I believe the first one identified is RedRock) don't have an integrase, but exist as an extra-chromosomal inhabitant of a bacterial cell. In order to be propagated, a partitioning system is utilized. See this paper for details: Dedrick RM, Mavrich TN, Ng WL, Cervantes Reyes JC, Olm MR, Rush RE, Jacobs-Sera D, Russell DA, Hatfull GF. Function, expression, specificity, diversity and incompatibility of actinobacteriophage parABS systems. Mol Microbiol. 2016 Aug;101(4):625-44. doi: 10.1111/mmi.13414. Epub 2016 Jun 10. PMID: 27146086; PMCID: PMC4998052. |
