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Recent Activity
All posts created by debbie
| Link to this post | posted 02 Feb, 2024 13:17 | |
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Hi Adam, This is not a gap-filling exercise. There is great coding potential. If I am at the correct gene (you didn't provide coordinates), there is a 31 base overlap between the gene that starts at 44145 and the next one that ends at 44114. Not that troublesome in this context. Just as important is that there are interesting hits to the gene that ends at 44114. More investigation is needed but I would want to know if I can make a 'toxin' or a DNA binding functional assignment. |
Posted in: Annotation → Gap or overlap in Superstar (BD2)
| Link to this post | posted 01 Feb, 2024 17:11 | |
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Hi Jacob, 1. Please send me the file from one of the computers. (not from your computer.) 2. What version of Windows are you using? My guess is that those computers are missing a driver or help file. 3. Check the DNA Master folder, where the drivers are stored. Check to see if the files listed match yours.) debbie |
Posted in: DNA Master → Numbering on Frames Page
| Link to this post | posted 01 Feb, 2024 15:11 | |
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Hi Arturo, the answer to this one is found here: https://seaphages.org/forums/topic/5207/ Please don't call it based on the data that is showing up at this point. Best, debbie |
Posted in: Cluster EC Annotation Tips → Potential minor capsid protein
| Link to this post | posted 31 Jan, 2024 02:04 | |
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afreise Not anymore. Approved Function now states to use DeepTMHMM. |
Posted in: Annotation → Membrane proteins
| Link to this post | posted 31 Jan, 2024 02:00 | |
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Kurt, only DeepTMHMM is used. Please see the January Faculty Meeting forum post for more information. https://seaphages.org/forums/topic/5612/ debbie |
Posted in: Functional Annotation → Membrane protein
| Link to this post | posted 30 Jan, 2024 21:25 | |
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Jacob, Hmmm. I am not sure. Is it a color thing? Go to preferences and change the colors and see if that fixes it. Are there "names" in the feature table of the DNA Master file? (that is where the numbers come from.) debbie |
Posted in: DNA Master → Numbering on Frames Page
| Link to this post | posted 29 Jan, 2024 03:57 | |
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Hi Chris, I went looking for the tRNA using Aragorn. It did not call it. I should have done my due diligence! Holly - check out this paper for consideration: Bacteriophage tRNA-dependent lysogeny: requirement of phage-encoded tRNA genes for establishment of lysogeny https://pubmed.ncbi.nlm.nih.gov/38236026/ I am not suggesting that your genome has a legit tRNA, but this paper describes the first case that we have published where a tRNA is found in the reverse direction while a protein coding gene is in the forward direction. Great classroom conversation! debbie |
Posted in: Phamerator → Missing gene in PhagesDB draft genome
| Link to this post | posted 26 Jan, 2024 20:17 | |
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Hi Holly, I don't believe it skipped a number, but rather called something that could not be demonstrated in phamerator. But i don't know what that is. It is not uncommon that numbers of genes don't match between your annotation and what is posted on phamerator. what is posted on phamerator is also what 'feeds' what is posted on phagesDB, so they will indeed match. Hence, I request that one never uses gene numbers (but rather use stops) to identify what gene you are working on. i hope that helps, debbie |
Posted in: Phamerator → Missing gene in PhagesDB draft genome
| Link to this post | posted 24 Jan, 2024 00:49 | |
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Amanda, I see no canonical slippage in RomansRevenge either. The real trick is that I could believe that the "T" portion of a tail assembly chaperone is present, but there is really no way to call it without a useful start codon. debbie BTW The gene that precedes the tail assembly chaperones has hits to minor capsid, HK97_gp 10 and minor tail protein. While I am confident that this protein is structural, it is not clear whether it is part of the head or the tail, please call it Hypothetical Protein. |
Posted in: Singleton Annotation Tips → tail assembly chaperones
| Link to this post | posted 20 Jan, 2024 23:28 | |
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Fred, i haven't looked at this specifically, but the cluster F1s are notorious for having MANY tiny genes that have the 1-4bp overlap with the preceding and next genes. So there are calls 'without' coding potential. As you have mentioned, it just makes sense to call them. Please remember that coding potential is not the end-all. The math (and remember that coding potential is an interpolated markov model - a mathematical calculation - that may or may not hold true when applied to small genes. debbie |