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All posts created by debbie

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Link to this post \| posted 15 Sep, 2015 11:36
debbie	Hi Greg, So without seeing, I can only guess. If you have no plaques with D29, it could be that you are not growing smeg. Streak out the liquid culture. If there are ANY colonies in 24 hrs., it is not smeg - or the smeg is contaminated. So to be thorough, culture everything to be sure nothing is contaminated. Start your smeg cultures from a single colony and follow regular protocols.

Posted in: Phage Discovery/Isolation → Smeg grown in 7H9 Complete W/ or W/O Tween for enrichment???

Link to this post \| posted 06 Sep, 2015 01:06
debbie	Hi Greg. Tween can also be used by the bacteria as a carbon source, so it will be broken down by the time you plate for phage. I would not fret too much about using a Tween culture for enrichment. Sharon is correct that Tween can inhibit phage adsorption, but once it is hydrolyzed, there should be no worries. debbie Hatfull Lab

Posted in: Phage Discovery/Isolation → Smeg grown in 7H9 Complete W/ or W/O Tween for enrichment???

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