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Recent Activity
All posts created by DanRussell
Link to this post | posted 24 Feb, 2016 20:35 | |
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Jeffrey Lawrence is aware of the issue and is working on it now. If he comes up with a fix and/or if there's anything to report, we'll let you know as soon as we do! –Dan |
Posted in: DNA Master → BLAST in DNAM
Link to this post | posted 24 Feb, 2016 14:49 | |
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Hi Keith et al, Yes, I'm seeing some slower peformance for sure in the VM DNA Master. For some functions, it's quite quick, but for others it drags. I hit a snag when I tried to import a fasta file that was about 170 kb long. It froze, and never made it through. I tried repeating, adjusting VM memory, etc., but nothing seemed to help. That would obviously be a dealbreaker, but perhaps we can figure out some workarounds or improvements. The 2017 SEA VM will use the newer version of Ubuntu, so perhaps those upgrades will help. I'm curious as to whether some of the problems might relate to the artificial PC file system that's set up by wine, and whether we can change some settings to improve along those lines. –Dan |
Posted in: DNA Master → Beta Test: DNA Master within the SEA VM
Link to this post | posted 24 Feb, 2016 14:45 | |
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Joseph Ross Hi Joe, Those Xs represent a "region of low complexity". What that basically means is that the amino acids there are quite similar to one another, or there are repetitive patterns, and as such they're bait for lots of meaningless alignments. Here's a link to NCBI's explanation. It's not something you should worry about, though! –Dan |
Posted in: DNA Master → Odd BLASTp alignment result
Link to this post | posted 24 Feb, 2016 14:39 | |
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jparkerjparker Hi Jordan, You can't just wait the 2.4 years for this result? Seriously, though, it looks like we're having the same issue. We'll let you know if we figure anything out. –Dan |
Posted in: DNA Master → BLAST in DNAM
Link to this post | posted 18 Feb, 2016 16:00 | |
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GregFrederick@letu.eduDan Russell I did end up taking a quick look in Phamerator, and it seemed like the Final genomes all had removed that reverse-strand gene, while the Draft ones still had it. So probably dropping it is the way to go. –Dan |
Posted in: Gene or not a Gene → Cluster G genes spanning the COS site
Link to this post | posted 16 Feb, 2016 21:12 | |
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coomansr Hi Roy, I think just going with the two recommended databases is fine. –Dan |
Posted in: Functional Annotation → HHPred databases
Link to this post | posted 12 Feb, 2016 22:16 | |
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Hi all, If you've successfully set up the SEA VM, but are still struggling with DNA Master, you might be interested in giving this new approach a shot. It allows you to run DNA Master within the SEA VM itself. It should be emphasized that this is a beta test only and is only recommended if you're an intrepid type and not worried about potential hiccups. That said, this has a lot of potential to be the future of how SEA-PHAGES uses DNA Master, and so any feedback we get would be welcome. I'll post a link to the instructions for doing this. Please use this forum to document any issues with the installation and/or running of DNA Master within the VM. http://seaphages.org/media/docs/DNA_Master_on_SEAVM_instructions.docx (Big thanks to Kristen Butela, Tammy Adair, Keith Hutchison, and others who have been troubleshooting non-Windows DNA Master.) Thanks, –Dan |
Posted in: DNA Master → Beta Test: DNA Master within the SEA VM
Link to this post | posted 12 Feb, 2016 15:08 | |
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Hi Greg, The coding potential looks sparse on the reverse strand in that region. Quick question: what are the other genomes where that reverse-strand gene is called? Are any (or all) of those still in "_Draft" form? –Dan |
Posted in: Gene or not a Gene → Cluster G genes spanning the COS site
Link to this post | posted 12 Feb, 2016 14:58 | |
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andersonr24@gator.uhd.edu Yep, just sent them to you. –Dan |
Posted in: SEA-PHAGES Virtual Machine → Student download of 2016 VM
Link to this post | posted 11 Feb, 2016 14:46 | |
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(Also, make sure you restart Phamerator once the database update is complete.) |
Posted in: Phamerator → Seemingly "uneven" updating of Phamerator db