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Recent Activity
All posts created by welkin
| Link to this post | posted 07 May, 2019 19:50 | |
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Hi All, The frameshift should be in close proximity to the C-terminus of the G protein; the GN-GK one is simply too far away. We probably should look at all the CZ4s and see if there is a consensus. W |
Posted in: Frameshifts and Introns → Cluster CZ4 Frameshift
| Link to this post | posted 30 Apr, 2019 15:07 | |
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Depends on how humid the air is. Under vaccuum and with dessicant, months; under humid conditions, maybe a few days tops. |
Posted in: Phage Discovery/Isolation → EM Sample Stability
| Link to this post | posted 29 Apr, 2019 14:57 | |
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I think this has to be a minor tail protein; otherwise there won't be enough proteins to make up the tail tip. |
| Link to this post | posted 26 Apr, 2019 18:32 | |
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Hi Dave, Sounds like it to me. Thanks! |
| Link to this post | posted 25 Apr, 2019 22:33 | |
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Hi Veronique, How long is the protein, and how many other minor tail proteins have you identified already? |
| Link to this post | posted 25 Apr, 2019 22:25 | |
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It is OK to indicate on your coversheet which scoring matrix and spacing matrix you used, as long as you don't change it on a per gene basis. Z-score and final score should still be included with every gene. Yes/no indicates if it is the best score or not, as compared to the other choices for that gene. |
Posted in: DNA Master → RBS Data
| Link to this post | posted 19 Apr, 2019 21:52 | |
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The capsid, scaffolding, and protease are fused into a single gene in the EEs and should be annotated as "major capsid and protease fusion protein" |
Posted in: Cluster EE Annotation Tips → capsid and protease fusion
| Link to this post | posted 18 Apr, 2019 13:09 | |
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Hi Sally, I thought M23 was on the list already– go head and use it. and I don't think we can be more specific and use M23B without better data. Best, Welkin |
Posted in: Functional Annotation → Lysin A in Rhodococcus phage
| Link to this post | posted 17 Apr, 2019 17:17 | |
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As of April 2019, we have been unable to identify the major capsid protein, and so you do not need to include it in your annotation. |
Posted in: Cluster EK Annotation Tips → major capsid protein
| Link to this post | posted 17 Apr, 2019 13:22 | |
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Hi Sally– Debbie and I spoke offline a bit, and we are going to amend the "RexA" only function. We try hard not to just use the protein names because students get stuck in the alphabet soup when there is no additional information (RecA and RexA are very different, no?) So I've added a bit to the designations: RexA family abortive infection protein RexB family abortive infection protein Thanks! Welkin |
Posted in: Functional Annotation → RexAB systems