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All posts created by welkin

| posted 25 Apr, 2018 13:17
You are not off the hook for the frameshift just because your phage is a Singleton. Chances are, you can find the tape measure in a flexible tailed genome, and the two genes right upstream are good candidates for the chaperones. Look for a slippery sequences near the end of the first one— a string of 5 or 6 Gs, a string of 5 or 6 As, three As or Gs followed by three of something else, XXXYYZZ, where X is A or G, etc.
Send us an email if you need help.
Posted in: Singleton Annotation Tipstail assembly chaperones
| posted 24 Apr, 2018 15:38
Cluster BI phages contain two genes that appear to encode the major tail protein.
Posted in: Cluster BI Annotation Tipstwo major tail genes
| posted 24 Apr, 2018 15:35
BG phages have a single endolysin, it should be labled "lysin A".
Posted in: Cluster BG Annotation Tipslysin A
| posted 24 Apr, 2018 15:33
The tape measure protein is not the longest protein in the genome, and it is located in an atypical region– amidst the genes that encode proteins found in the capsid.
Posted in: Cluster BG Annotation Tipstape measure
| posted 24 Apr, 2018 15:32
BF phages have a large cassette of tRNAs, which will not be drawn on a Phamerator map. It will look like a gap in coding genes.
Posted in: Cluster BF Annotation TipstRNAs
| posted 24 Apr, 2018 15:31
lysin A is found after the terminase and before the portal protein.
Posted in: Cluster BF Annotation Tipslysin A
| posted 24 Apr, 2018 15:30
the tape measure is amidst the proteins that comprise the capsid, rather than following them.
Posted in: Cluster BF Annotation Tipstape measure
| posted 24 Apr, 2018 14:38
the terminase proteins are located near the center of the genome, downstream from all the minor tail proteins.
Posted in: Cluster BE Annotation Tipsterminase
| posted 24 Apr, 2018 14:30
The immunity repressor is located just after the minor tail proteins and lysin, and the integrase is locate far downstream.
Posted in: Cluster BD Annotation Tipsimmunity repressor
| posted 24 Apr, 2018 14:26
BD phages have a single endolysin, it should be labeled "lysin A".
Posted in: Cluster BD Annotation Tipslysin A