SEA-PHAGES Logo

The official website of the HHMI Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science program.

Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.

Alpha-L-arabinofuranosidase B?

| posted 25 Apr, 2019 20:48
Annotating Begonia, a CV phage.
The homolog of gene 33 (27738-28667) has been called Alpha-L-arabinofuranosidase in Lucky 10, but that is not on the approved function list.
Based on location - just after a minor tail - and based on HHPred matches to Alpha-L-arabinofuranosidase, which has binding domain to arabinose as well as catalytic activity, call function minor tail protein?
The Begonia protein matches this enzyme with 99.8%, covering 60% of aa sequence, and very low <10^-24 E-value
| posted 25 Apr, 2019 22:33
Hi Veronique,
How long is the protein, and how many other minor tail proteins have you identified already?
| posted 25 Apr, 2019 23:50
309 amino acids (930 bp including stop codon)
I have identified two minor tail proteins before the lysis cassette and three after .
This would be a 4th and last minor tail after the lysis cassette
| posted 29 Apr, 2019 14:57
I think this has to be a minor tail protein; otherwise there won't be enough proteins to make up the tail tip.
 
Login to post a reply.