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Recent Activity
All posts created by nietof
| Link to this post | posted 24 May, 2019 16:34 | |
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Hi We are working on Snazzy. The tail assembly chaperones are genes 20 and 21 both on the forward strand but gene 20 ORF is in the 1 and 21 in the 3 frame. We located a GGGGGAA slippery sequence at 15336 in 20. We thought the ribosome would read the GGG at position 15337-15339 in frame 1 for a G and then slip back and read again GGA in frame 3 for another G. It yields 266 residues. When I blastp the product after the frameshift it aligns 100%, 100% coverage and 99.6% identity(residue 69 is different) to Violet tail assembly chaperone protein with no gaps. The sequence in the region where the shift happens aligns perfectly with that of Violet. Am I looking at this right? Is it a -1 frameshift but the ribosome jumps from frame 1 to 3. Thank you Fernando |
| Link to this post | posted 12 Jul, 2018 14:15 | |
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Hi folks I am working with a group of energetic highschoolers from LI, NY. They are interested in testing host range for their phage isolates using smeg. How broad is the range of bacterial hosts they can test? Should they stick to closely related bacteria? Thank you Fernando |
Posted in: Host-Range Project → Basic Host Range Project Information