Link to this post | posted 19 Feb, 2021 23:38

nietof

Hey Retaking this thread. We are annotating Sarma624. Gene 35 blasted in phagesdb gets two hits with a PLA2-like protein domain function, Bigphil_35 and Mozy_42. It is a phospholipase A2 It doesn't appear in the list of accepted functions yet. Most of the other F1 phages have NKF for the same gene. The HHPred hits are all human actin binding protein or other not close to a phospholipase. I would be inclined to think BigPhil and Mozy's annotations are incorrect and thus to call it NKF for Sarma.

Link to this post | posted 05 Jan, 2021 15:07
nietof	Debbie Thank you, I will try that, and Happy New Year!!! F

Link to this post | posted 05 Jan, 2021 12:38

nietof

I am running DMA Master in windows 10. Set it up all preferences according to latest instructions. I am able to autoannotate my genome. I ran the BLAST all genes overnight and it doesn't complete. DNA Master becomes unresponsive and the only way to get out is by ending it using task manager. This is the first time I am running DNA Master in windows 10 and it is a brand new laptop. Thank you Fernando

Link to this post | posted 09 May, 2020 20:52
nietof	Hi Dan THank you. Trust all is well with you the UPitt folks and your families. Best F

Link to this post | posted 09 May, 2020 16:58
nietof	Hi The entry for Temperate shows either true or false, but in some instances the entry for this column is none. Is none the same as false? thank you Fernando

Link to this post | posted 09 Mar, 2020 17:58
nietof	Hi What is the best way for downloading fasta files for a selected group of phages to build phylogenetic trees? I downloaded the complete fasta file from phagesdb and then I searched the sequences using the phage names, but it takes forever for large clusters like A1. Thank you Fernando

Link to this post | posted 21 Jan, 2020 16:00
nietof	Hi Dan I am using a school laptop from home, the laptop was initially set up by our IT. I am working using an administrator account. I found a post on DNA Master and Windows 10 from Welkin dealing with the Windows 10 UAC. I follow her instructions to add DNA Master as a trusted application in windows 10. I reinstalled DNA Master. Let's see how long it works. Thank you F

Link to this post | posted 21 Jan, 2020 14:52

nietof

Hi I installed DNA Master in a PC with windows 10. It works fine for a while then it doesn't find the path and the application doesn't open up at all. The icon in the desktop appears white. When I try to open the application from the actual folder where it is installed, the DNAMas icon also appears white and it gives me the following error: "Windows cannot access the specified device, path, or file. You may not have the appropriate permissions to access the item". This is the second time it happens. The first time I uninstalled it and install it again. Any clues? thanks Fernando

Link to this post | posted 05 Dec, 2019 21:52
nietof	Not sure why the smiley appears there the error number is 2108. thanks F

Link to this post | posted 05 Dec, 2019 21:44
nietof	Hi All of a sudden today I am getting an error when I open DNA Master "Queries table is corrupt. Reload from FTP DNA Master FTP site. When I try to reload from FTP site it gives me a second error "An error occurred when attempting to initialize the Borland Database Engine (Error $210". Any helps is appreciated. Fernando