Link to this post | posted 11 May, 2018 12:16

Tamarah_Adair

There are 103 genes for Sonali. When we BLAST we receive 20 results and the rest of the genes return "There are no BLAST results for this feature". The BLAST freezes before it finishes and we have tried it several times. The report that eventually populates has lines like the following. Is this a complete BLAST that just has very few hits? Why are the feature numbers greater than our feature list? Thanks! Sent 25 requests (25 total) to the QBlast server. Checking for completed results at 9:33:06 PM… Error (12) retrieving results for RID FA02Z622015 for feature 110 Error (12) retrieving results for RID FA034F2E015 for feature 112 Error (12) retrieving results for RID FA03AAG9014 for feature 115 Error (12) retrieving results for RID FA03FMB7014 for feature 116 Error (12) retrieving results for RID FA03MXTY015 for feature 125 Error (12) retrieving results for RID FA03VVRG015 for feature 126 Error (12) retrieving results for RID FA0409U7015 for feature 127 Error (12) retrieving results for RID FA045PJ8014 for feature 128 Error (12) retrieving results for RID FA04BUFP014 for feature 129 Error (12) retrieving results for RID FA04GD2D014 for feature 130 Error (12) retrieving results for RID FA04NZ27014 for feature 131 Error (12) retrieving results for RID FA04VYNG014 for feature 132 Error (12) retrieving results for RID FA050CWU015 for feature 133 Error (12) retrieving results for RID FA0551M0015 for feature 134 Error (12) retrieving results for RID FA05B09Z015 for feature 135 Error (12) retrieving results for RID FA05GD74015 for feature 136 Error (12) retrieving results for RID FA05P9X5014 for feature 137 Error (12) retrieving results for RID FA061R6R014 for feature 139 Error (12) retrieving results for RID FA06HHWZ014 for feature 142 Error (12) retrieving results for RID FA06PCEC014 for feature 143 20 results read; still waiting for 5 results… Continuing to send queries at 9:46:44 PM… Sent 25 requests (50 total) to the QBlast server. Checking for completed results at 9:48:53 PM… Error (12) retrieving results for RID FA10M2XR014 for feature 146 Error (12) retrieving results for RID FA10TKEV014 for feature 147 4.62Mb

Link to this post | posted 11 May, 2018 12:26
welkin	Hi Tammy, I have never seen that error before. I recommend trying a partial batch BLAST– 10 to 20 genes or so at a time, and then saving after each successful one. Good luck! Welkin

Link to this post | posted 11 May, 2018 12:42
Tamarah_Adair	When I BLAST 1 gene at a time, I get OLE 8040152. How do you limit the BLAST to a set of genes? Thanks.

Link to this post | posted 11 May, 2018 13:36
Tamarah_Adair	Also, my previous question about entering the locus tags, was not about where to enter, but about adding the underscore. The underscore _ will not enter in the locus tag box, so it enters as SEAELESAR instead of SEA_ELESAR. Just checking the details…thanks.

Link to this post | posted 11 May, 2018 13:42
Tamarah_Adair	And..I found the instructions in the new guide for BLASTING a subset. I'll try that.

Link to this post | posted 11 May, 2018 15:31
welkin	Great! Check the override box to the right of the locus tag field and you should be able to enter the underscore.

Link to this post | posted 11 May, 2018 16:30
Tamarah_Adair

Link to this post | posted 12 May, 2018 12:07
Tamarah_Adair	Welkin Pope Hi Tammy, I have never seen that error before. I recommend trying a partial batch BLAST– 10 to 20 genes or so at a time, and then saving after each successful one. Good luck! Welkin Is anyone else having this problem? I tried several different sets of genes and even another genome and I keep getting this error. Did something change on the server settings? 38Kb

Link to this post | posted 12 May, 2018 12:07
Tamarah_Adair	Welkin Pope Hi Tammy, I have never seen that error before. I recommend trying a partial batch BLAST– 10 to 20 genes or so at a time, and then saving after each successful one. Good luck! Welkin Is anyone else having this problem? I tried several different sets of genes and even another genome and I keep getting this error. Did something change on the server settings? 38Kb

Link to this post | posted 12 May, 2018 13:40
welkin	Are you using the HTTPS setting to connect to NCBI? Have you tried the same file on a different computer?