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All posts created by Tamarah_Adair

| posted 24 Jul, 2018 17:44
Thanks
Posted in: PECAANNew Features in PECAAN
| posted 24 Jul, 2018 15:50
Hi Claire,
I have used Pecaan to annotate Sonali. This is the first time I have used it and I am now exporting the files. Is there a way to place the Function in the Function box for the complete annotations?
Thank you,
Tammy
Posted in: PECAANNew Features in PECAAN
| posted 11 Jun, 2018 20:37
We have tested these primers specific for the tapemeasure protein for Arthrobacter phages isolated on ATCC 21022.
Posted in: ArthrobacterArthrobacter Cluster specific primers
| posted 14 May, 2018 20:43
HMM. Thanks. I just tried a gene from Sonali and got the OLE 80040152 error.
Posted in: DNA MasterDNAMaster BLAST failure
| posted 14 May, 2018 12:27
Yea. I am using the SEAPHAGE connection in the same way that we have used all semester. A few weeks ago were able to BLAST ELESAR. I tried a couple of genomes on several computers and then asked my TA to try on her PC. She got the same errors. She was going to reinstall DNAMaster and try again. Are you able to BLAST within DNAMAster?
Posted in: DNA MasterDNAMaster BLAST failure
| posted 12 May, 2018 12:07
Welkin Pope
Hi Tammy,
I have never seen that error before. I recommend trying a partial batch BLAST– 10 to 20 genes or so at a time, and then saving after each successful one.

Good luck!
Welkin

Is anyone else having this problem?
I tried several different sets of genes and even another genome and I keep getting this error. Did something change on the server settings?
Posted in: DNA MasterDNAMaster BLAST failure
| posted 12 May, 2018 12:07
Welkin Pope
Hi Tammy,
I have never seen that error before. I recommend trying a partial batch BLAST– 10 to 20 genes or so at a time, and then saving after each successful one.

Good luck!
Welkin

Is anyone else having this problem?
I tried several different sets of genes and even another genome and I keep getting this error. Did something change on the server settings?
Posted in: DNA MasterDNAMaster BLAST failure
| posted 11 May, 2018 16:30
smile
Posted in: DNA MasterDNAMaster BLAST failure
| posted 11 May, 2018 13:42
And..I found the instructions in the new guide for BLASTING a subset. I'll try that.
Posted in: DNA MasterDNAMaster BLAST failure
| posted 11 May, 2018 13:36
Also, my previous question about entering the locus tags, was not about where to enter, but about adding the underscore. The underscore _ will not enter in the locus tag box, so it enters as SEAELESAR instead of SEA_ELESAR. Just checking the details…thanks.
Posted in: DNA MasterDNAMaster BLAST failure