Link to this post | posted 11 Oct, 2019 15:35
cellokiwi	Hi Vic, Sorry for the delay. Attached is a picture that has the plate the day we first looked at it next to how it appeared after maybe 2 weeks in the fridge. It is so weird. It will only let me add one attachment to the post so I will put a second with a picture of the streak plate. 2.00Mb

Link to this post | posted 09 Oct, 2019 01:44
cellokiwi	I will snap a picture of the streak plate in the am and post it.

Link to this post | posted 09 Oct, 2019 00:56

cellokiwi

Hello Hive Mind, We are having a rather weird problem that has persisted for a few weeks. We started the semester with Arthrobacter growing well. It formed beautiful lawns, we had the majority of students with plaques, some progressing as far as flooding a webbed plate. They just needed to start titering. Then things suddenly stopped working. We can't get a nice lawn anymore to save our lives. We have tried refreshing the LB plates, the top agar, the CaCl2, all of it. We went back to the first streak plate (that worked initially), didn't work. Went back to the sample sent from Pitt, didn't work. I'm running out of ideas as to why something that worked beautifully before is suddenly crashing and burning. Weird thing #2: Some students that put their plates with plaques in the refrigerator (wrapped in parafilm) went back to check them and the lawn was disintegrated to the point that you could no longer see the plaques. What is UP with this organism? Any and all tips/help is GREATLY appreciated!

Link to this post | posted 22 Aug, 2019 21:00
cellokiwi	Students can't find the app in the Apple AppStore or the Google Playstore. Did it get taken down?

Link to this post | posted 19 Jun, 2019 17:53
cellokiwi	Thank you so much! Miriam is listed as the primary contact so I've given her a heads up that you guys will probably be emailing her. Everything else on the institution page is correct and good to go.

Link to this post | posted 17 Jun, 2019 18:03
cellokiwi	I would like to start using A. globiformis as the host for phage hunting this fall semester. Since this organism is not in ATCC, as far as I can tell, how do I get a starter culture? Thanks!

Link to this post | posted 14 Jan, 2019 16:42
cellokiwi	We are trying to calculate burst size for our phage but are having trouble getting a one step growth curve to work reliably. Is there a popular protocol that people use with smeg that works well? Thanks!