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Recent Activity
All posts created by GregFrederick@letu.edu
| Link to this post | posted 08 Sep, 2015 17:31 | |
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I may be missing this. But everywhere I have looked I just see "Agar Plate" listed. QUESTION: So from the Resource Guide I cannot really tell. The only agar listed in the Appendix is for Luria Agar and Top Agar (1x and 2x). Is the "Bottom Agar" when plating for plaques actually Luria agar or is it Middlebrook 7H9 Complete with CaCl2 and 1.5% Agar or what? I'm guessing it is the 7H9 complete agar. But I want to optimize our chances for success. It may be in the resource guide somewhere. But I'm not finding it. Thanks for being out there! Greg 
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Posted in: Phage Discovery/Isolation → Plaque Bottom Agar - Recipe???
| Link to this post | posted 07 Sep, 2015 22:58 | |
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Thanks Ladies! We should know shortly. Hopefully we will still see phage after the enrichment step. I'll let you know. The plaque plating media will not have Tween in it. So that should help with the absorption step. Hopefully. Greg |
| Link to this post | posted 04 Sep, 2015 17:49 | |
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It may be in the Resource guide. But I just saw this line on the Complete with Tween recipe. "Do not add Tween 80 to media if the bacteria being grown will be used for phage isolation or propagation." So my questions are these: Can the 5ml of O.N. smeg culture that is used in the enrichment process be grown with Tween added? The 7H9 medium used during the enrichment will not have Tween. I just want to make certain that the trace Tween in the host smeg volume should not have too much negative impact. What about the smeg mixed with the top agar for plating phage? Please let me know ASAP. We are inoculating today. Thanks. |