Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.
Recent Activity
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Frameshifts and Introns
While frameshifts and introns are likely used by phages in other genes, we do not have any wet bench evidence to support their existence in any other genes at this time. Therefore, there should not be any other frameshifts in the annotation other than in the tail assembly chaperones, and there should not be any other introns other than in the Cluster J phages similar to LittleE or BAKA. If you have strong evidence for an intron ( a disruption in an essential gene, like the capsid or portal) please post!
If you want to know if your phage has a findable frameshift, please also check the cluster specific annotation forums before posting.