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TAC frameshifts in CF

Link to this post \| posted yesterday, 21:39
rdejong	We have a possible Tail Assembly Chaperone frameshift in two new cluster CF phages (CurlyFries and RegnumDei). The only possible slippery sequence is GGATTTC. In two previous CF annotations that have this same sequence (Shagrat and Madraxi), a frameshift was not called. In another CF annotation, Jflix2, the putative slippery sequence is GGGTTTC, and it was called as a frameshift. This sequence does have two places for slippage as opposed to one. Seems like the GGATTTC, conserved in 4 phages in the cluster, could also be a slippery sequence. But without stronger evidence, better to not call it as a frameshift? Any opinions out there? Thanks!

Link to this post | posted yesterday, 21:39

rdejong

We have a possible Tail Assembly Chaperone frameshift in two new cluster CF phages (CurlyFries and RegnumDei).

The only possible slippery sequence is GGATTTC. In two previous CF annotations that have this same sequence (Shagrat and Madraxi), a frameshift was not called.

In another CF annotation, Jflix2, the putative slippery sequence is GGGTTTC, and it was called as a frameshift. This sequence does have two places for slippage as opposed to one.

Seems like the GGATTTC, conserved in 4 phages in the cluster, could also be a slippery sequence. But without stronger evidence, better to not call it as a frameshift?

Any opinions out there?

Thanks!