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Joseph Stukey posted in Numbering on Frames Page
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Frameshift in a singleton, OnionKnight
| Link to this post | posted 12 Apr, 2023 17:36 | |
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My class and I are working on OnionKnight, a singleton that came from another institution. Genes 15/16 have all the appropriate features associated with being the tail assembly chaperone ORFs, but there's nothing to compare it with. Gene 15 runs from 9987 to 10592, and there's a conspicuous GGGAAA from 10484 to 10489, although it's followed not by a purine but by a C. I don't see anything else that screams "here's the frameshift." Is it safe to assign 10487 as the location of a -1 frameshift, or should I just skip it and leave the second ORF alone? |
| Link to this post | posted 13 Apr, 2023 00:57 | |
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Mitch, That is sufficient data to call the -1 frameshift. Best, debbie |
| Link to this post | posted 13 Apr, 2023 00:59 | |
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Thanks, Debbie! -Mitch |