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All posts created by lhughes
| Link to this post | posted 16 Sep, 2015 16:52 | |
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Hi All: Bekah - we've been using spread plates with liquid cultures of Streptomyces instead of the spore/top agar method at UNT. Steve got his protocols from me, so I'm to blame  I sent him the following information by e-mail this morning about how we get good lawns with this method: We usually grow up 50 mL culture until turbid (for a subculture usually 2 days, for an initial culture from spores usually 4 days). Then we centrifuge in a 50 mL conical at 3000rpm (Sorvall tabletop with buckets), pour off all but about 10 mL of the supernatant, and resuspend. 100 uL of this usually gives a nice confluent lawn. Lee |
Posted in: Streptomyces → Strep problems