The official website of the HHMI Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science program.

Welcome to the forums at Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at

Adding Illumina reads

| posted 07 Nov, 2015 21:08
I have tried to add Illumina reads to an assembly using "addSolexaReads.perl" and following Dan's instructions. However I keep getting the following error "-ace must be specified". Everything looks correct to me with the command and filenames.

Any suggestions?

| posted 08 Nov, 2015 03:03
Figured it out! I was using the old form, not the newer form of the command (forgot to read the comments at the bottom of the video).
| posted 10 Nov, 2015 18:30
Yep, you got it Lee. I made the original video with an older version of consed. The new syntax should be:

addSolexaReads.perl -ace your_ace_file.ace -fastqfof reads_file.fof -fasta fasta_file.fof

Edited 10 Nov, 2015 18:31
Login to post a reply.