SEA-PHAGES | All posts created by viknesh

Link to this post \| posted 27 Sep, 2016 18:42
viknesh	Christine, While you are doing your tests, it may be worth asking for smeg from your buddy school. That way, you'll at the very least have a strain to compare to. Vic

Posted in: Mycobacterium → Growth of msmeg on agar plates

Link to this post \| posted 22 Sep, 2016 21:09
viknesh	Hi Christine, It's going to be hard to tell what you have without photos of your plates with colonies. I've attached a figure with smeg on streak plates over time when incubated at 37C. You'll see from the figure that small specks become visible at day 2, and they gradually grow into larger well-defined colonies with a dry and irregular morphology by day 4 - 5. They are white to tan, depending on the age of the colonies and the properties of the plate. An important test for you to perform is a plaque assay (or a spot test) with D29. If you see plaques or a cleared spot on your bacterial lawn, then you can be confident that you are working with smeg. Vic 686Kb

Link to this post | posted 22 Sep, 2016 21:09

viknesh

Hi Christine,

It's going to be hard to tell what you have without photos of your plates with colonies.
I've attached a figure with smeg on streak plates over time when incubated at 37C. You'll see from the figure that small specks become visible at day 2, and they gradually grow into larger well-defined colonies with a dry and irregular morphology by day 4 - 5. They are white to tan, depending on the age of the colonies and the properties of the plate.

An important test for you to perform is a plaque assay (or a spot test) with D29. If you see plaques or a cleared spot on your bacterial lawn, then you can be confident that you are working with smeg.

Vic

Posted in: Mycobacterium → Growth of msmeg on agar plates

Link to this post \| posted 07 Jul, 2016 21:26
viknesh	Hi Emily, I've seen this happen before - lawns and cultures of Arthrobacter take an extra day or two to become dense or saturate, and lawns tend to be thin and spotty/clumpy. We found that this happened unexpectedly from time to time when we grew cells at 30C. Once we switched to doing everything at 26C, this problem went away. I suggest starting from your glycerol stock and try to do everything (streak, culture in liquid, plate and incubate) at 26C (or at room temperature). I'll be curious to know if this solves the problem for you too. Vic

Link to this post | posted 07 Jul, 2016 21:26

viknesh

Hi Emily,

I've seen this happen before - lawns and cultures of Arthrobacter take an extra day or two to become dense or saturate, and lawns tend to be thin and spotty/clumpy. We found that this happened unexpectedly from time to time when we grew cells at 30C. Once we switched to doing everything at 26C, this problem went away. I suggest starting from your glycerol stock and try to do everything (streak, culture in liquid, plate and incubate) at 26C (or at room temperature). I'll be curious to know if this solves the problem for you too.

Vic

Posted in: Arthrobacter → Cell growth issues

Recent Activity

All posts created by viknesh