Link to this post | posted 03 Mar, 2020 20:43
stpage	Yeah, that location in Mariokart is just AGGGCCG…. Yago84 did not call the frameshift but there is a aagggcct So maybe I should just follow that.

Link to this post | posted 03 Mar, 2020 17:15
stpage	Thanks again, Erin. It makes more sense looking at the GenBank entry rather than Phamerator. I don't have that many Gs, but I'll try a mockup of the frameshift and see how it looks in Mariokart.

Link to this post | posted 02 Mar, 2020 01:48
stpage	Not annotating frameshift in absence of wet bench evidence, GC-rich region but not obvious slippery sequence. Some in cluster have two tail assembly chaperone back to back in same frame (Sour, NHagos), most overlapping in separate frames (Cloverminnie, Mariokart).

Link to this post | posted 23 May, 2019 22:05
stpage	The CP is almost nonexistent, and it is less than 2/3 the length of the BE ones, though. Blasting the first primase of a BD (Alvy) vs the putative first primase of the BE is 30%coverage 35% aa alignment, which is intriguing not very convincing… THe BLAST alignment for that ORF is about the same with Karimac, your BD phage, too… Edited 23 May, 2019 22:11

Link to this post | posted 23 May, 2019 01:38
stpage	What about BE phages? They have a similar pattern. Several BE have a gene upstream of primase that overlaps with only fair coding potential (e.g., Karimac) that Glimmer and GM don't like. Can we call that DNA primase, too?

Link to this post | posted 11 Apr, 2017 18:14
stpage	The last couple of days, I can't open the Lawrence Lab web page to download DNA master…

Link to this post | posted 06 Oct, 2016 17:09
stpage	Thanks, Dan. (And, the direct link to SEAVM helped a lot too)

Link to this post | posted 15 Sep, 2016 17:51
stpage	Any Mac people who can help me with some basic WINE setup? Shallee pages@franklinpierce.edu