Link to this post | posted 27 Aug, 2018 15:58
kaylafast	P0FF streak from stock #2 Incubation: 37 degrees C, 4 days Edited 27 Aug, 2018 16:08 1.75Mb

Link to this post | posted 27 Aug, 2018 15:55
kaylafast	P2FF streak from stock #1 Incubation: 37 degrees C, 2 days (1 day photo unavailable) Edited 27 Aug, 2018 16:07 2.23Mb

Link to this post | posted 27 Aug, 2018 15:52
kaylafast	P1FF streak from stock #1 Incubation: 37 degrees C, 2 days (1 day photo unavailable) Edited 27 Aug, 2018 16:06 2.26Mb

Link to this post | posted 27 Aug, 2018 15:51
kaylafast	P0FF Streak from stock #1 Incubation: 37 degrees C, 5 days Edited 27 Aug, 2018 16:01 1.95Mb

Link to this post | posted 27 Aug, 2018 15:28

kaylafast

We are having difficulty getting D29 and other control phages to appear on our M. smeg plates. If you look hard enough and get a lot of light coming through the plate, we can see clearings on spot tests and plaques on plaque assays. But, we have to look really hard, and most photographs cannot capture that the plaques were ever there. I've started multiple cultures from single colonies on a streak plate, and feel that all media components are good (many have been remade). The streak plates have come from different glycerol stocks. I have read the suggestion to streak out the liquid culture and if there are any colonies in 24 hrs, it is not smeg - or the smeg is contaminated. Does this mean any growth at all within 24 hours or just individual colonies? Streaks from our P1FF and P2FF do show growth within 24 hours, but just smears not individual colonies. Attached is a picture of a D29 plaque assay of the 10-3 dilution showing the plaques. I have many more pictures of streak plates and phage test plates that I can share if they will be helpful. 139Kb

Link to this post | posted 26 Apr, 2018 14:27
kaylafast	Thanks Welkin!

Link to this post | posted 26 Apr, 2018 13:21
kaylafast	Thanks, Debbie! I will be at the symposium/faculty meeting, so I will see you then.

Link to this post | posted 25 Apr, 2018 19:02

kaylafast

I am having trouble saving the results after Blasting all genes within DNA Master. I've blasted whole genomes in DNA Master many times before and successfully saved the results within the Blast tab. For the current genome I've blasted several times, see the blast data, but then they disappear when I reopen the saved file. As a test, I blasted one gene. When selecting how many hits to save for that one gene, all results for all genes were temporarily back. When I reopened the file all blast hits were gone again. I'm attaching a DNA Master file that has had the whole genome blasted, but I can't view blast results. Am I missing some step to save the results or a step to view them. My DNA Master is up to date. Thanks! 1.39Mb

Link to this post | posted 19 Apr, 2018 15:34
kaylafast	Thanks!

Link to this post | posted 18 Apr, 2018 16:24

kaylafast

In our C1 phage Cane17 we are seeing about equal support for the functions RNA ligase and phosphoesterase. There are several members of C1 that support each function. Any suggestions of which to choose or how to choose? Our evidence for each function is below. F: RNA ligase SIF-BLAST: RNA ligase, phagesDB & NCBI, Yucca gp 95 (10 others), AOZ62967.1, 1:1, 95%, e-102 SIF-HHPred: RNA ligase LigT, HHPred, d1iuha_, 57.9%, 99.57% SIF-Syn: RNA ligase; Lukilu & Phox F: phosphoesterase SIF-BLAST: phosphoesterase, phagesDB & NCBI, Pleione gp98 (5 others), AEN79717.1, 1:1, 95%, -102 SIF-HHPred: 2H phosphoesterase/ligase, HHPred, 5LDQ_C, 81.1%, 99.5% SIF-Syn: phosphoesterase; ZaigoTaiga Cane17 gp89 MEFVADDRIPPGYHIPSSWMLRSADAEQAKQTGGMVALYPSTESAQKIVVPGGEPIDDLHLTVTYFGQDVTGQDPTELVDFLYYLAPQFPPIEANIFGTAVFNSTGEDPCIVYLVGNSPHLTPLFQQLKRFALEHYPGAAEQHDPWIPHITAAYGSGAMIDYEGPVVFDRIGIRWPGADQDFNLZ