Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.
Recent Activity
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Frameshift K6
| Link to this post | posted 20 Sep, 2020 19:03 | |
|---|---|
|
Identified the frameshift but I am unsure if its -1 or +2. Yellow highlight, frame 1, AAs: IEGK then it should jump to frame 3 (soon to be blue highlight) and continue AA: ISQV It seems to me that the slipper sequence "GGGAAAA" final "A" 11670 is counted 2x. The final "A" in codon for amino acid "K" and again as the first "A" in codon for amino acid "I" I though this is the definition of a -1. However, when I learned how to read the 6 different frames I thought moving down 2 frames is a +2? |
208Kb| Link to this post | posted 20 Sep, 2020 19:16 | |
|---|---|
|
We have more experimental data for -1 frameshifts. So we will call the -1 until we can do an experiment to differentiate. debbie |
| Link to this post | posted 20 Sep, 2020 19:18 | |
|---|---|
|
|
okay, thanks for the quick reply! |