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Debbie Jacobs-Sera posted in Whole phage starterator reports
cdshaffer posted in Whole phage starterator reports
Shallee Page posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
Debbie Jacobs-Sera posted in Plaques appear on spot test, don't appear in titer
How many holins?
| Link to this post | posted 09 Aug, 2017 16:30 | |
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I am looking at cluster J phages and there are instances where two holins have been called. Allowed? The structure is LysA-holin-LysB-holin. |
| Link to this post | posted 10 Aug, 2017 14:37 | |
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David, Which phages have the second potential holins? The cluster J phages I've looked at have pham 5845 between the two lysins, which does not look like a holin via HHPred (attached) and does not seem to have a transmembrane region by TMHMM? Karen |
129Kb| Link to this post | posted 10 Aug, 2017 14:46 | |
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Karen, That is indeed the "holin" I am looking at in Dmpstrdiver. It does not really seem like one to me based on all the evidence we have but I was wondering if there was evidence I don't know about for why this has been called a holin. Best, Dave |