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The official website of the HHMI Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science program.

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Phage Discovery/Isolation

Topics Posts Views Last post
A atrocyaneus DNA extraction
2 8 Mar 30, 2024
10:59 PM
Top Agar contamination
6 47 Mar 18, 2024
10:53 AM
Plaques appear on spot test, don't appear in titer
1 9 Feb 16, 2024
9:01 AM
Best nuclease mix?
2 15 Dec 5, 2023
1:52 PM
Precipitation of CaCl2 in PYCA top-agar
5 49 Oct 6, 2023
11:12 AM
Contamination of top agar and possibly other components pages: 1 2
16 120 Oct 3, 2023
2:22 PM
Electron Microscopes
8 54 Jun 21, 2023
2:36 PM
Confirmation of A. globiformis strain sent from Pitt
1 24 Jun 13, 2023
8:52 AM
Low plate titer issues
6 27 Apr 6, 2023
1:27 PM
Protocol to grow phage in culture?
6 84 Dec 5, 2022
12:17 AM
DNA Extraction Troubleshooting - Can we skip the nuclease treatment?
6 53 Nov 12, 2022
2:21 PM
Peptone question
4 32 Sep 28, 2022
2:34 PM
2X smeg top agar
2 21 Sep 21, 2022
1:09 PM
New hosts - human microbiome pages: 1 2
11 99 Sep 13, 2022
9:22 AM
260/280 ratios too high from DNA extraction
1 19 Jul 19, 2022
2:18 PM
Electron microscopy protocols
3 28 May 5, 2022
11:06 PM
Taking images of plaques
2 13 Mar 30, 2022
3:08 PM
Capsid denaturation
3 19 Oct 27, 2021
7:34 PM
Low isolation rate for Gordonia terrae this semester
2 25 Oct 23, 2021
12:09 PM
Syringe filter failure?
4 33 Sep 27, 2021
10:56 AM

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