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Recent Activity
cdshaffer posted in Whole phage starterator reports
fbaliraine posted in Phage DoRead Start at 52714 bp with 8 bp overlap vs start at 52718 with 4 bp overlap?
Debbie Jacobs-Sera posted in Phage DoRead Start at 52714 bp with 8 bp overlap vs start at 52718 with 4 bp overlap?
Reviving
| Link to this post | posted 03 Mar, 2025 19:41 | |
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Hi is there a standard protocol for reviving phage from -80 stocks? Well, I assume there is.  What I mean is, what do y'all recommend?
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| Link to this post | posted 03 Mar, 2025 19:57 | |
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Shallee, in a perfect world. you can take 1ul of the sample, make some dilutions, and plate for single plaques. If that doesn't work, there are some additional considerations. (and of course you can start with more than 1ul.) Second step is to pick a single plaque and make a new working lysate, being sure to verify (maybe by pcr) that you have the phage you are interested in. debbie |