Recently, Eric Miller at NC State asked me about phage genome ends, and how a 3' overhang (sticky end) would look in an assembled genome compared to how a 5' overhang would look. So I made a picture! Hopefully it explains how you might diagnose either of these cases from sequencing reads, and how you ...
Posts tagged: Sequencing
3' versus 5' overhangs in sequencing data
Written by on Oct. 31, 2014 .
Free Sequencing: We need your DNA!
Written by on Oct. 23, 2014 .
Well, not YOUR DNA...
If you've been phage hunting with a host that's IN the order Actinomycetales, but is NOT Mycobacterium smegmatis mc2 155, the Hatfull lab would like to sequence your phages' genomes free of charge. So that means if you have DNA for phages found on Rhodococcus, Arthrobacter, Streptomyces ...
Count on me: Entering Institutional Data into seaphages.org
Written by on Oct. 9, 2014 in Using seaphages.org.
Hopefully, everyone in the SEA-PHAGES community is well into the swing of the new semester. Now is a great time to start collecting some institution-specific data and aggregating it into program-wide numbers. In the next couple of days, an email will be sent to SEA-PHAGES faculty inviting them to begin using seaphages.org and entering relevant ...
Sequencing Deadlines and Information
Written by on Oct. 6, 2014 .
This year, all "official" (HHMI-supported) SEA-PHAGES sequencing will take place at the University of Pittsburgh. Because schools on the quarter system begin their semesters later, we have two separate deadlines for DNA submission this year.
Deadline for Semester Schools
Please send your genomic DNA so that it arrives in Pittsburgh by November 21, 2014. ...