This year, all "official" (HHMI-supported) SEA-PHAGES sequencing will take place at the University of Pittsburgh. Because schools on the quarter system begin their semesters later, we have two separate deadlines for DNA submission this year.
Deadline for Semester Schools
Please send your genomic DNA so that it arrives in Pittsburgh by November 21, 2014.
Deadline for Quarter Schools
Please send your genomic DNA so that it arrives in Pittsburgh by December 19, 2014.
Guidelines for DNA
We can't use samples that are resuspended in TE. The EDTA interferes with enzymatic shearing of the DNA. You can resuspend your DNA in Elution Buffer (Tris) or in purified water.
We need a minimum of 5 micrograms of genomic DNA.
At least 50 ng/µl. Ideal is 100-300 ng/µl. If you're above 300 ng/µl please consider diluting to a workable concentration. Keep in mind that spec-based quantifications (like Nanodrop) count all absorbance at 260 nm, not just genomic DNA, so they often overestimate the real concentration. Err on the high side of the amount of DNA you send.
Please submit–either via email to email@example.com or in the shipping box–an image of the gDNA being run on an agarose gel. We use these to look for sample integrity (not already sheared/degraded) and purity (no RNA).
344 Crawford Hall
Department of Biological Sciences
4249 Fifth Avenue
Pittsburgh, PA 15260