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The official website of the HHMI Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science program.

Sequencing Deadlines and Information

This year, all "official" (HHMI-supported) SEA-PHAGES sequencing will take place at the University of Pittsburgh.  Because schools on the quarter system begin their semesters later, we have two separate deadlines for DNA submission this year.

Deadline for Semester Schools

Please send your genomic DNA so that it arrives in Pittsburgh by November 21, 2014.

Deadline for Quarter Schools

Please send your genomic DNA so that it arrives in Pittsburgh by December 19, 2014.

Guidelines for DNA

Buffer

We can't use samples that are resuspended in TE.  The EDTA interferes with enzymatic shearing of the DNA.  You can resuspend your DNA in Elution Buffer (Tris) or in purified water.

Amount

We need a minimum of 5 micrograms of genomic DNA.

Concentration

At least 50 ng/µl.  Ideal is 100-300 ng/µl.  If you're above 300 ng/µl please consider diluting to a workable concentration.  Keep in mind that spec-based quantifications (like Nanodrop) count all absorbance at 260 nm, not just genomic DNA, so they often overestimate the real concentration.  Err on the high side of the amount of DNA you send.

Gel Picture

Please submit–either via email to cab106@pitt.edu or in the shipping box–an image of the gDNA being run on an agarose gel.  We use these to look for sample integrity (not already sheared/degraded) and purity (no RNA).

Shipping Address

Charlie Bowman

344 Crawford Hall

Department of Biological Sciences

4249 Fifth Avenue

Pittsburgh, PA 15260

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Current Events

Phage Discovery Workshop 12A

June 22, 2019 to June 28, 2019

Upcoming Events

Phage Discovery Workshop Option B

July 6, 2019 to July 12, 2019

Phage Discovery Workshop 12B

July 7, 2019 to July 13, 2019

Virtual Site-Visit with SEA Team

September 1, 2019 to October 31, 2019

Recent Events

2019 Faculty Workshop

June 9, 2019 to June 12, 2019

2019 SEA GENES Faculty Meeting

June 9, 2019 to June 11, 2019

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Welkin Pope posted in HNH vs methylase in Cluster J phages.

Welkin Pope posted in Finding introns

Welkin Pope posted in HNH vs methylase in Cluster J phages.

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