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The official website of the HHMI Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science program.

Posts tagged: Finishing

3' versus 5' overhangs in sequencing data

3' versus 5' overhangs in sequencing data

Recently, Eric Miller at NC State asked me about phage genome ends, and how a 3' overhang (sticky end) would look in an assembled genome compared to how a 5' overhang would look.  So I made a picture!  Hopefully it explains how you might diagnose either of these cases from sequencing reads, and how you ...

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