Recently, Eric Miller at NC State asked me about phage genome ends, and how a 3' overhang (sticky end) would look in an assembled genome compared to how a 5' overhang would look. So I made a picture! Hopefully it explains how you might diagnose either of these cases from sequencing reads, and how you ...
Posts tagged: Finishing
3' versus 5' overhangs in sequencing data
Written by on Oct. 31, 2014 .