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All posts created by westoverk

Link to this post \| posted 22 Nov, 2019 18:14
westoverk	Thanks Debbie!

Posted in: Cluster EA Annotation Tips → frame shift

Link to this post \| posted 22 Nov, 2019 13:54
westoverk	Hi Welkin, We are annotating one EA5 (Nebulous)phage and one EA11 (Ixel) phage. They are both very similar to Neferthena (EA5). Neferthena called a frameshift for the tail chaperones. We find the same slippery set of 6 guanines in both of our sequences. Other closely related phages did not call the shift and left the genes separate (MCubed, Finny, Sansa). Recommendations? Thanks for your help. Kristi Edited 22 Nov, 2019 16:50

Link to this post | posted 22 Nov, 2019 13:54

westoverk

Hi Welkin,
We are annotating one EA5 (Nebulous)phage and one EA11 (Ixel) phage. They are both very similar to Neferthena (EA5). Neferthena called a frameshift for the tail chaperones. We find the same slippery set of 6 guanines in both of our sequences. Other closely related phages did not call the shift and left the genes separate (MCubed, Finny, Sansa). Recommendations? Thanks for your help.
Kristi

Edited 22 Nov, 2019 16:50

Posted in: Cluster EA Annotation Tips → frame shift