SEA-PHAGES | All posts created by crump.katie

Link to this post \| posted 01 May, 2023 13:57
crump.katie	Thanks Joe and Debbie. Yes, originally, we did not record any functions in the function fields via the Description tab. I like students to keep all that in an Excel file. However, I added it in manually and then moved it and cleared the fields as noted in directions of preparing the minimal file for final submission. Thanks! I just couldn't remember if I added the function in the description tab in the past one by one. For some reason, I thought it populated from the BLAST and was lost for a second of how to get them added before moving them. But, Joe cleared it up yesterday…Thanks again!

Link to this post | posted 01 May, 2023 13:57

Thanks Joe and Debbie. Yes, originally, we did not record any functions in the function fields via the Description tab. I like students to keep all that in an Excel file. However, I added it in manually and then moved it and cleared the fields as noted in directions of preparing the minimal file for final submission. Thanks! I just couldn't remember if I added the function in the description tab in the past one by one. For some reason, I thought it populated from the BLAST and was lost for a second of how to get them added before moving them. But, Joe cleared it up yesterday…Thanks again!

Posted in: DNA Master → Function field

Link to this post \| posted 30 Apr, 2023 20:08
crump.katie	I am preparing my final files for submission and I re-blasted the entire genome. However, about 9 genes are not blasting within DNA Master. I even just BLASTed the single gene in DNA Master and after 20 minutes…still nothing and it appears to just stop. When I copy the sequence into NCBI BLAST on the website directly, I get hits immediately. I'm not sure why this is happening. I didn't have these issues in January when I BLASTed the entire genome then. Not sure what I should do about this for the final submission files… Edited 30 Apr, 2023 20:10

Link to this post | posted 30 Apr, 2023 20:08

crump.katie

I am preparing my final files for submission and I re-blasted the entire genome. However, about 9 genes are not blasting within DNA Master. I even just BLASTed the single gene in DNA Master and after 20 minutes…still nothing and it appears to just stop. When I copy the sequence into NCBI BLAST on the website directly, I get hits immediately. I'm not sure why this is happening. I didn't have these issues in January when I BLASTed the entire genome then. Not sure what I should do about this for the final submission files…

Edited 30 Apr, 2023 20:10

Posted in: DNA Master → DNAMaster BLAST failure

Link to this post \| posted 30 Apr, 2023 16:06
crump.katie	I have a similar issue/question. In preparing the final file for submission, the function field is blank from the beginning. Therefore, when I go to prepare the minimal file, there is nothing to copy and paste and then delete. Also, as Joe commented, I don't remember inserting the functions one by one and then moving them according to the final file instructions. Am I missing something? Do I leave it blank? Thanks for your help! - Katie

Link to this post | posted 30 Apr, 2023 16:06

crump.katie

I have a similar issue/question. In preparing the final file for submission, the function field is blank from the beginning. Therefore, when I go to prepare the minimal file, there is nothing to copy and paste and then delete. Also, as Joe commented, I don't remember inserting the functions one by one and then moving them according to the final file instructions. Am I missing something? Do I leave it blank? Thanks for your help! - Katie

Posted in: DNA Master → Function field

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