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All posts created by Bowen.Meng

| posted 28 Feb, 2024 15:14
Hi Kristen,

Thank you for your input. We will keep the these two genes separate unless there is further evidence to call it with a programmed frameshift.
Posted in: Frameshifts and IntronsFrameshift in CZ4 Phage Annalisa
| posted 27 Feb, 2024 17:09
Hi,

We are currently annotating CZ4 phage Annalisa (https://phagesdb.org/phages/Annalisa/)and have come across a potential programmed frameshift of the Tail Assembly Chaperone (Gene 15 and 16 in the gene list). When I look at other phages in this cluster, it seems the shift occurs with the sequence GGGGACT. Annalisa however, does not have this sequence in the similar location as other CZ4 phages like Oregano and Sekhmet. Instead, it seems like the sequence in Annalisa is GGCGACT. Is the frameshift not present in Annalisa, or is there another possibility for a slippery sequence? I've attached an image of the region for Annalisa.
Edited 27 Feb, 2024 17:10
Posted in: Frameshifts and IntronsFrameshift in CZ4 Phage Annalisa