Link to this post | posted today, 13:27

ACMPhageHunters

Allegany College of Maryland is a small 2-year college in the beautiful Appalachian mountains. The area has affordable housing (it really does!). - Michèle Barmoy Here are the details: The following position has been posted and will close on October 26, 2025, at 11:59 PM. Faculty, Tenure Track - Biology Link to posting: https://www.schooljobs.com/careers/allegany/jobs/5094621/faculty-tenure-track-biology?pagetype=jobOpportunitiesJobs Individuals interested in the position must apply online at the link above by the deadline. Faculty will be responsible for teaching lecture and laboratory sections of at least two of the following: various human anatomy and physiology courses (e.g., musculoskeletal, A&P I and II) and various biology courses (e.g., general biology, human biology); microbiology may be included in responsibilities depending on qualifications. Please reach out to an HR Team Member if you have any questions about the position. Shanese Westfall, SHRM-CP HR Generalist Human Resources and Payroll she/her/hers Allegany College of Maryland College Center Room #168A 12401 Willowbrook Road, SE Cumberland, MD 21502 T: 301-784-5231 F: 301-784-5058 www.allegany.edu/hr Edited today, 16:22

Link to this post | posted 08 Feb, 2024 21:42

ACMPhageHunters

I am a student of Profesor Barmoy. I am having trouble calling a function for a gene of a Siphoviridae phage. In HHpred, I have hits for a base plate structural protein in a myoviride phage. I also have hits in HHpred for a minor tail protein. My results include no collagen-like or glycine-rich proteins to assist in calling a minor tail, but also have no directions on calling a base plate protein. Can anyone assist in which to call and why? Thank you in advance.

Link to this post | posted 08 Feb, 2024 21:41

ACMPhageHunters

I am a student of Profesor Barmoy. I am having trouble calling a function for a gene of a Siphoviridae phage. In HHpred, I have hits for a base plate structural protein in a myoviride phage. I also have hits in HHpred for a minor tail protein. My results include no collagen-like or glycine-rich proteins to assist in calling a minor tail, but also have no directions on calling a base plate protein. Can anyone assist in which to call and why? Thank you in advance.

Link to this post | posted 08 Feb, 2024 21:23

ACMPhageHunters

I am a student of Professor Baromy. I am having trouble calling the function of a protein. My matches on HHpred include both Holliday junction resolvase and RuvC. Per the calling instructions, I am to call a Holliday junction resolvase only if not RusA or RuvC. The way that I am interpreting this information is that I would call this gene a RuvC because my HHpred matches include more than just Holiday junction resolvase. Would my assumption be correct? I appreciate your time and help. -Brandon

Link to this post | posted 08 Feb, 2024 21:22

ACMPhageHunters

I am a student of Professor Baromy. I am having trouble calling the function of a protein. My matches on HHpred include both Holliday junction resolvase and RuvC. Per the calling instructions, I am to call a Holliday junction resolvase only if not RusA or RuvC. The way that I am interpreting this information is that I would call this gene a RuvC because my HHpred matches include more than just Holiday junction resolvase. Would my assumption be correct? I appreciate your time and help. -Brandon

Link to this post | posted 12 Apr, 2023 20:31
ACMPhageHunters	Adam and Chris, Thank you for your prompt replies. My background is no where close to molecular biology and I'm learning as I go, I don't know what a sigma factor is and didn't know that it had anything to do with an HTH. But now I know and I can add this topic to my summer reading! Thanks again for your kind help. Michèle

Link to this post | posted 11 Apr, 2023 18:34

ACMPhageHunters

Hello, I'm having trouble with two gene function calls regarding HTH domains. In the first gene, many of the Phagesdb Blast hits call a "helix-turn-helix DNA binding domain protein" and exact matches on the NCBI results are "HTH DNA binding protein", but I don't see any references to an HTH binding domain in the HHpred or CDD results. The second gene has similar results. I don't want to just go with the crowd on these, so without HHpred or CDD confirmation, I'm leaning towards NKF. Is there anything else I should do to check for these HTH domains? The genes in question: StolenFromERC Cluster: E PECAAN # 32 Start 29,140 Stop: 28955 Reverse PECANN #130 Start: 68,963 Stop: 68700 Reverse Thanks for your help, Michèle