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Randall DeJong posted in HTH or no HTH
Steve Cresawn posted in How to cite...version number for Phamerator
Debbie Jacobs-Sera posted in deoxycytidylate deaminase OR nucleoside deoxyribosyltransferase.
cdshaffer posted in deoxycytidylate deaminase OR nucleoside deoxyribosyltransferase.
Debbie Jacobs-Sera posted in deoxycytidylate deaminase OR nucleoside deoxyribosyltransferase.
HTH or no HTH
| Link to this post | posted yesterday, 22:52 | |
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We were annotating the following protein, with hits to binding proteins and HTH domain containing proteins. MPLVKTAAEINREIRSMCPPTAPPPPPASRSDRQLGPMQYALLELVRSKPYWVVDRAFWRVMARKLRVPAWRVRKVFLSLEEGGYILVSERENPRIAQATERGRR Checking for HTH on HHpred output wasn't totally clear. In the output below, I've put green boxes around possible HTH regions for both the Query and the Target. The Target has a definite HTH domain. The Query, however, is a little doubtful. One of the helices is only 3 amino acids long, and my understanding is that at least 5 aa's are needed for a stable helix. Rick Pollenz was consulted and confirmed that there is not a HTH in that protein. He used two programs to investigate: https://users.cis.fiu.edu/~giri/bioinf/GYM2/prog.html https://npsa-prabi.ibcp.fr/cgi-bin/npsa_automat.pl?page=/NPSA/npsa_hth.html Thanks Rick! Our call is going to be DNA binding protein |
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