SEA-PHAGES | Frameshift in a singleton, OnionKnight

Frameshift in a singleton, OnionKnight

Link to this post \| posted 12 Apr, 2023 17:36
balish	My class and I are working on OnionKnight, a singleton that came from another institution. Genes 15/16 have all the appropriate features associated with being the tail assembly chaperone ORFs, but there's nothing to compare it with. Gene 15 runs from 9987 to 10592, and there's a conspicuous GGGAAA from 10484 to 10489, although it's followed not by a purine but by a C. I don't see anything else that screams "here's the frameshift." Is it safe to assign 10487 as the location of a -1 frameshift, or should I just skip it and leave the second ORF alone?

Link to this post | posted 12 Apr, 2023 17:36

My class and I are working on OnionKnight, a singleton that came from another institution. Genes 15/16 have all the appropriate features associated with being the tail assembly chaperone ORFs, but there's nothing to compare it with. Gene 15 runs from 9987 to 10592, and there's a conspicuous GGGAAA from 10484 to 10489, although it's followed not by a purine but by a C. I don't see anything else that screams "here's the frameshift." Is it safe to assign 10487 as the location of a -1 frameshift, or should I just skip it and leave the second ORF alone?

Link to this post \| posted 13 Apr, 2023 00:57
debbie	Mitch, That is sufficient data to call the -1 frameshift. Best, debbie

Link to this post \| posted 13 Apr, 2023 00:59
balish	Thanks, Debbie! -Mitch

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Frameshift in a singleton, OnionKnight