SEA-PHAGES | Disinfection Debate: does the order matter?

Link to this post \| posted 10 Sep, 2021 16:36
c.sunnen	So after running this course for 6+ years, I discovered that my colleague and I clean-up our benches differently at the end of lab, and we both swear we were taught (during the same training) the way we each do it is "correct." Whenever disinfecting BEFORE lab, we always do Ci-decon first, let it dry, followed by 70% Ethanol. The question is whether the order should be reversed at the end of experiments, before leaving the lab. Should it remain Ci-decon first, then EtOH? Or should it be EtOH first, followed by Ci-decon? Does it matter? (and if it does matter, can you share why?)

Link to this post | posted 10 Sep, 2021 16:36

So after running this course for 6+ years, I discovered that my colleague and I clean-up our benches differently at the end of lab, and we both swear we were taught (during the same training) the way we each do it is "correct."

Whenever disinfecting BEFORE lab, we always do Ci-decon first, let it dry, followed by 70% Ethanol. The question is whether the order should be reversed at the end of experiments, before leaving the lab.

Should it remain Ci-decon first, then EtOH? Or should it be EtOH first, followed by Ci-decon? Does it matter?

(and if it does matter, can you share why?)

Link to this post \| posted 10 Sep, 2021 17:55
viknesh	c.sunnen So after running this course for 6+ years, I discovered that my colleague and I clean-up our benches differently at the end of lab, and we both swear we were taught (during the same training) the way we each do it is "correct." Whenever disinfecting BEFORE lab, we always do Ci-decon first, let it dry, followed by 70% Ethanol. The question is whether the order should be reversed at the end of experiments, before leaving the lab. Should it remain Ci-decon first, then EtOH? Or should it be EtOH first, followed by Ci-decon? Does it matter? (and if it does matter, can you share why?) We typically do EtOH after CiDecon so that we remove from the bench residual phenol from the cidecon.

Link to this post | posted 10 Sep, 2021 17:55

viknesh

c.sunnen
So after running this course for 6+ years, I discovered that my colleague and I clean-up our benches differently at the end of lab, and we both swear we were taught (during the same training) the way we each do it is "correct."

Whenever disinfecting BEFORE lab, we always do Ci-decon first, let it dry, followed by 70% Ethanol. The question is whether the order should be reversed at the end of experiments, before leaving the lab.

Should it remain Ci-decon first, then EtOH? Or should it be EtOH first, followed by Ci-decon? Does it matter?

(and if it does matter, can you share why?)

We typically do EtOH after CiDecon so that we remove from the bench residual phenol from the cidecon.

Link to this post \| posted 10 Sep, 2021 18:25
c.sunnen	And this is why we love you, Vic. You have all the answers! Thanks for clarifying this for us! I hope it's useful for others, too! Nikki

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Disinfection Debate: does the order matter?