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JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
RyanWheeler posted in Plaques appear on spot test, don't appear in titer
LysinK-like, CHAP domain
Link to this post | posted 17 Jun, 2019 02:26 | |
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Gene 65 in BossLady (42154,43032) and its homolog in Zartrosa show nice HHPred matches to the lytic CHAPK domain of the endolysin LysK from Staphylococcus aureus bacteriophage K among others. I attach snapshots from HHPred matches. The matches cover the length of the protein with over 99% prob and low E-values. Pasting info from HHPred hits from BossLady (uploaded in Pecaan) 4CT3_C ORF30/ORF32; VIRAL PROTEIN, PEPTIDOGLYCAN, PROTEASE; HET: EPE, GOL, CMH, MMC; 1.69A {STAPHYLOCOCCUS PHAGE K} 99.7 46.5753 26 165 1 137 9.2e-18 5UDN_B Phage-associated cell wall hydrolase; lysin, d-alanyl-l-alanine endopeptidase, CHAP domain; HET: GOL; 1.971A {Streptococcus pyogenes MGAS5005} 99.6 62.3288 1 203 2 184 4.8e-16 Can go with more generic hydrolase but PDB files suggest we can be more specific. |