Welcome to the forums at seaphages.org. Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at info@seaphages.org.
Recent Activity
Alpha-L-arabinofuranosidase B?
Link to this post | posted 25 Apr, 2019 20:48 | |
---|---|
|
Annotating Begonia, a CV phage. The homolog of gene 33 (27738-28667) has been called Alpha-L-arabinofuranosidase in Lucky 10, but that is not on the approved function list. Based on location - just after a minor tail - and based on HHPred matches to Alpha-L-arabinofuranosidase, which has binding domain to arabinose as well as catalytic activity, call function minor tail protein? The Begonia protein matches this enzyme with 99.8%, covering 60% of aa sequence, and very low <10^-24 E-value |
Link to this post | posted 25 Apr, 2019 22:33 | |
---|---|
|
Hi Veronique, How long is the protein, and how many other minor tail proteins have you identified already? |
Link to this post | posted 25 Apr, 2019 23:50 | |
---|---|
|
309 amino acids (930 bp including stop codon) I have identified two minor tail proteins before the lysis cassette and three after . This would be a 4th and last minor tail after the lysis cassette |
Link to this post | posted 29 Apr, 2019 14:57 | |
---|---|
|
I think this has to be a minor tail protein; otherwise there won't be enough proteins to make up the tail tip. |