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JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
JustinA posted in Plaques appear on spot test, don't appear in titer
Viknesh Sivanathan posted in Plaques appear on spot test, don't appear in titer
RyanWheeler posted in Plaques appear on spot test, don't appear in titer
new type of nucleotide pyrophosphohydrolase
Link to this post | posted 23 Aug, 2018 18:10 | |
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Annotation of phage Gilgamesh, gene 43 (27658-28101) in PECAAN. This prediction shows 99.4% prob, with ~90% query and subject coverage HHPRED hit to MutT/NUDIX type nucleotide pyrophosphohydrolase. There is an approved term for "MazG-like nucleotide pyrophosphohydrolase". According to this paper (The Journal of Biological Chemistry 286, 30691-30705) the nucleotide pyrophosphohydrolases fall into"structurally different superfamilies, such as the MutT-related hydrolases (Nudix) (α + β), dUTPase (all-β), dITPase (Maf/HAM1) (α/β), all-α-NTP pyrophosphatases (MazG), and phospho-ribosyl-ATP pyrophosphatase (HisE)."The MazG type are a superfamily made up of all alpha helices as can been seen in this PDB crystal structure of the eponymous MazG protein 3CRA. The MutT (also called Nudix type) show a kind of sandwich with a beta sheet between alpha helices like 4KYX I would propose adding either "MutT-like nucleotide pyrophosphohydrolase" to keep a parallel structure to the other entry or just have a generic "nucleotide pyrophosphohydrolase" at the bottom of the list for those enzymes that are not in the MazG superfamily. |
Link to this post | posted 23 Aug, 2018 18:13 | |
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sounds good. I'll add both. |