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elongation factor P

Link to this post \| posted 01 Aug, 2018 20:14
washj	Request for new function - elongation factor or translation elongation factor P to be more specific (Saguaro_77). Appprox 15 hits (> 90% probability and >92% coverage) in HHPRED to tranlation elongation factor P or elongation factor 5A which is the eukaryotic homolog. EF-P stimulates ribosomal peptidyltransferase activity/prevents ribosome stalling and is highly conserved.

Link to this post \| posted 07 Aug, 2018 16:16
welkin	so in this particular case, the Saguaro gene is not long enough to be a full EF-P. It should be around 180aa, and this is only 107. EF-P was crystallized in sections, and this appears to be aligning to the OB domain, which is a beta-barrel. We might add elongation factor P to the functional list at some point, but I don't think this gene has enough evidence. http://www.pnas.org/content/101/26/9595.long Edited 07 Aug, 2018 16:17

Link to this post | posted 07 Aug, 2018 16:16

welkin

so in this particular case, the Saguaro gene is not long enough to be a full EF-P. It should be around 180aa, and this is only 107. EF-P was crystallized in sections, and this appears to be aligning to the OB domain, which is a beta-barrel.
We might add elongation factor P to the functional list at some point, but I don't think this gene has enough evidence.

http://www.pnas.org/content/101/26/9595.long

Edited 07 Aug, 2018 16:17