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Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Annotating Cluster C1 phage onn Macbook Pro
| Link to this post | posted 04 Jun, 2018 18:16 | |
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Hello, Is there any way to get a large cluster C1 phage genome to annotate on a MacBook Pro (using WINE). Most of the time, I can open the file but when I try to show ORFs in a frames view I run out of memory or get various error messages. Another time I was able to generate the ORFs in Frames, but then could not get RBS scores when I selected a gene (again an error message). I will be using this computer during the upcoming faculty workshop, so any help would be much appreciated. Ann |
| Link to this post | posted 04 Jun, 2018 21:12 | |
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Hi Ann, I'm not sure there is. My recent experiences with WINE have varied, and almost always the size of the genome is a determining factor in how well WINE works. A 50 kb genome loads in a few seconds, but a 100 kb genome never works. Not helpful, obviously, but others may have better suggestions. You may be able to request a loaner laptop for the workshop. I'd ask Vic and/or Billy. –Dan |